{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:17:13Z","timestamp":1759637833753},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T00:00:00Z","timestamp":1464652800000},"content-version":"vor","delay-in-days":60,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hepatic fibropoiesis has been confirmed in canine visceral leishmaniasis. In fibrotic disease, hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>) play an important role in fibropoiesis, undergoing activation by <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 to acquire characteristics of myofibroblasts. These cells show extensive capacity for proliferation, motility, contractility, collagen synthesis and extracellular matrix component synthesis. The aim of this work was to identify markers of <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> activation in 10 symptomatic and 10 asymptomatic dogs naturally infected with <jats:italic>Leishmania (Leishmania) infantum<\/jats:italic>. Eight uninfected dogs were used as controls. Alpha\u2010actin (\u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>), vimentin and cytokeratin were investigated by immunohistochemistry as <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> markers. The cytokine <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 in tissue was also evaluated by immunohistochemistry. All infected dogs showed higher numbers of reticular fibres than controls. Fibropoiesis found in infected dogs was always associated with the presence of parasites and chronic granulomatous hepatitis. Positive correlation was found among fibropoiesis, parasite tissue load and expression of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>. There was no correlation between fibropoiesis, vimentin and cytokeratin markers. The expression of cytokine <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 was higher in infected dogs than in controls, but not significantly different between symptomatic and asymptomatic dogs. These results confirm previous work describing the intense hepatic fibropoiesis in dogs naturally infected with <jats:italic>Leishmania infantum,<\/jats:italic> but now associated them with overexpression of <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2, where \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> may be a superior marker for activated <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> cells in <jats:styled-content style=\"fixed-case\">CVL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/iep.12179","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T06:42:56Z","timestamp":1464676976000},"page":"139-149","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunohistochemical study of hepatic fibropoiesis associated with canine visceral leishmaniasis"],"prefix":"10.1111","volume":"97","author":[{"given":"Igor M. V. M.","family":"Madeira","sequence":"first","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debora. M. O.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline. A.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cesar A.","family":"Vilela","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izabela F. G.","family":"Amorim","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo V.","family":"Caliari","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina C.","family":"Souza","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wagner L.","family":"Tafuri","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,31]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"14","article-title":"Expression of Toll\u2010like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum","volume":"14","author":"Amorim I.F.","year":"2013","journal-title":"BMC Immunol."},{"key":"e_1_2_7_3_1","first-page":"259","article-title":"Some new aspects of the kala\u2010azar pathology. 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