{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T00:14:48Z","timestamp":1769818488015,"version":"3.49.0"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,9,23]],"date-time":"2016-09-23T00:00:00Z","timestamp":1474588800000},"content-version":"vor","delay-in-days":53,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007297","name":"International Islamic University Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007297","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Coeliac disease (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> toxic gluten peptides by <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DQ<\/jats:styled-content>2 or <jats:styled-content style=\"fixed-case\">DQ<\/jats:styled-content>8 molecules to gluten\u2010sensitive T cells. A \u03c9\u2010gliadin\/C\u2010hordein peptide (<jats:styled-content style=\"fixed-case\">QPFPQPEQPFPW<\/jats:styled-content>) and a rye\u2010derived secalin peptide (<jats:styled-content style=\"fixed-case\">QPFPQPQQPIPQ<\/jats:styled-content>) were proposed to be toxic in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>, as they yielded positive responses when assessed with peripheral blood T\u2010cell clones derived from individuals with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>. We sought to assess the immunogenicity of the candidate peptides using gluten\u2010sensitive T\u2010cell lines obtained from <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> small intestinal biopsies. We also sought to investigate the potential cross\u2010reactivity of wheat gluten\u2010sensitive T\u2010cell lines with peptic\u2013tryptic digested barley hordein (<jats:styled-content style=\"fixed-case\">PTH<\/jats:styled-content>) and rye secalin (<jats:styled-content style=\"fixed-case\">PTS<\/jats:styled-content>). Synthesised candidate peptides were deamidated with tissue transglutaminase (<jats:styled-content style=\"fixed-case\">tTG<\/jats:styled-content>). Gluten\u2010sensitive T\u2010cell lines were generated by culturing small intestinal biopsies from <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients with peptic\u2013tryptic gluten (<jats:styled-content style=\"fixed-case\">PTG<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">PTH<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">PTS<\/jats:styled-content>, along with autologous <jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>s for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of <jats:sup>3<\/jats:sup>H\u2010thymidine. The majority of T\u2010cell lines reacted to the peptides studied. There was also cross\u2010reactivity between wheat gluten\u2010sensitive T\u2010cell lines and the hordein, gliadin and secalin peptides. <jats:styled-content style=\"fixed-case\">PTH<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PTS<\/jats:styled-content>, barley hordein and rye secalin\u2010derived <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> antigen\u2010sensitive T\u2010cell lines showed positive stimulation with <jats:styled-content style=\"fixed-case\">PTG<\/jats:styled-content>. \u03c9\u2010gliadin\/C\u2010hordein peptide and rye\u2010derived peptide are immunogenic to gluten\u2010sensitive T\u2010cell lines and potentially present in wheat, rye and barley. Additional <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> toxic peptides may be shared.<\/jats:p>","DOI":"10.1111\/iep.12199","type":"journal-article","created":{"date-parts":[[2016,9,23]],"date-time":"2016-09-23T07:06:55Z","timestamp":1474614415000},"page":"303-309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Coeliac disease: immunogenicity studies of barley hordein and rye secalin\u2010derived peptides"],"prefix":"10.1111","volume":"97","author":[{"given":"Widya A.","family":"Wahab","sequence":"first","affiliation":[{"name":"Department of Biotechnology Kulliyyah of Science International Islamic University Malaysia (IIUM) Kuantan Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanja","family":"\u0160uligoj","sequence":"additional","affiliation":[{"name":"Division of Diabetes and Nutritional Sciences Department of Gastroenterology King's College London Rayne Institute St. Thomas\u2019 Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Ellis","sequence":"additional","affiliation":[{"name":"Division of Diabetes and Nutritional Sciences Department of Gastroenterology King's College London Rayne Institute St. Thomas\u2019 Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"C\u00f4rtez\u2010Real","sequence":"additional","affiliation":[{"name":"Division of Diabetes and Nutritional Sciences Department of Gastroenterology King's College London Rayne Institute St. Thomas\u2019 Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul J.","family":"Ciclitira","sequence":"additional","affiliation":[{"name":"Division of Diabetes and Nutritional Sciences Department of Gastroenterology King's College London Rayne Institute St. Thomas\u2019 Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.4.603"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(95)06220-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-009-0080-4"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-200605000-00005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.2.212"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-006-3108-0"},{"key":"e_1_2_9_8_1","first-page":"1649","article-title":"Oats induced villous atrophy in coeliac disease","volume":"52","author":"Lundin K.E.A.","year":"2003","journal-title":"J. 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