{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:36:26Z","timestamp":1788885386489,"version":"build-2803163510"},"reference-count":75,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T00:00:00Z","timestamp":1492992000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006143","name":"Department of Science and Technology, Government of Kerala","doi-asserted-by":"publisher","award":["SR\/CSI\/288\/2012(G)"],"award-info":[{"award-number":["SR\/CSI\/288\/2012(G)"]}],"id":[{"id":"10.13039\/501100006143","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Amino acid metabolism is a significant metabolic activity in humans, especially of sulphur\u2010containing amino acids, methionine and cysteine (Cys). Cys is cytotoxic and neurotoxic in nature; hence, mammalian cells maintain a constant intracellular level of Cys. Metabolism of Cys is mainly regulated by two thiol dioxygenases: cysteine dioxygenase (<jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content>) and 2\u2010aminoethanethiol dioxygenase (<jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content> are the only human thiol dioxygenases reported with a role in Cys metabolism and localized to mitochondria. This metabolic pathway is important in various human disorders, as it is responsible for the synthesis of antioxidant glutathione and is also for the synthesis of hypotaurine and taurine. <jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content> is the most extensively studied protein, whose high\u2010resolution crystallographic structures have been solved. As compared to <jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ADO<\/jats:styled-content> is less studied, even though it has a key role in cysteamine metabolism. To further understand <jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content>'s structure and function, the three\u2010dimensional structures have been predicted from I\u2010<jats:styled-content style=\"fixed-case\">TASSER<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SWISS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MODEL<\/jats:styled-content> servers and validated with <jats:styled-content style=\"fixed-case\">PROCHECK<\/jats:styled-content> software. Structural superimposition approach using <jats:styled-content style=\"fixed-case\">iPBA<\/jats:styled-content> web server further confirmed near\u2010identical structures (including active sites) for the predicted protein models of <jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content> as compared to <jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content>. In addition, protein\u2013protein interaction and their association in patho\u2010physiology are crucial in understanding protein functions. Both <jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content> interacting partner profiles have been presented using <jats:styled-content style=\"fixed-case\">STRING<\/jats:styled-content> database. In this study, we have predicted a 3D model structure for <jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content> and summarized the biological roles and the pathological consequences which are associated with the altered expression and functioning of <jats:styled-content style=\"fixed-case\">ADO<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CDO<\/jats:styled-content> in case of cancer, neurodegenerative disorders and other human diseases.<\/jats:p>","DOI":"10.1111\/iep.12222","type":"journal-article","created":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T03:02:49Z","timestamp":1493089369000},"page":"52-66","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Understanding human thiol dioxygenase enzymes: structure to function, and biology to pathology"],"prefix":"10.1111","volume":"98","author":[{"given":"Bibekananda","family":"Sarkar","sequence":"first","affiliation":[{"name":"Center for Animal Sciences School of Basic and Applied Sciences Central University of Punjab Bathinda Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh","family":"Kulharia","sequence":"additional","affiliation":[{"name":"Center for Computational Sciences School of Basic and Applied Sciences Central University of Punjab Bathinda Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil K.","family":"Mantha","sequence":"additional","affiliation":[{"name":"Center for Animal Sciences School of Basic and Applied Sciences Central University of Punjab Bathinda Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4310"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-014-1843-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1853201"},{"key":"e_1_2_10_5_1","first-page":"1192","article-title":"Sulfate metabolism is abnormal in patients with rheumatoid arthritis. 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