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Severe immune responses, excessive cellular recruitment and necrosis of cells all play a role in disease pathology. Understanding the pathogenesis of disease can aid in identifying potential new therapeutic targets or simply act as a diagnostic tool. <jats:italic>Burkholderia pseudomallei<\/jats:italic> is a Gram\u2010negative bacterium that can cause acute and chronic diseases. The <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mouse has been shown to be highly susceptible to aerosol challenge with <jats:italic>B.\u00a0pseudomallei<\/jats:italic> and hence acts as a good model to study the acute and potentially lethal form of the disease melioidosis. In our study, <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were challenged and culled at predetermined time points to generate a pathological time course of infection. Lung, liver and spleen were subjected to pathological and immunohistochemical analysis. The number and type of microscopic lesions within each organ, as well as the location and the mean percentage of neutrophils, B cells, T cells and <jats:italic>Burkholderia<\/jats:italic> capsule antigen within the lesions, were all characterized during the time course. Neutrophils were determined as the key player in tissue pathology and generation of lesions, with B cells playing an insignificant role. This detailed pathological assessment increases our understanding of <jats:italic>B.\u00a0pseudomallei<\/jats:italic> disease.<\/jats:p>","DOI":"10.1111\/iep.12258","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T04:43:52Z","timestamp":1515559432000},"page":"347-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Histopathological and immunohistochemical characterization of <i>Burkholderia pseudomallei<\/i> lesions in an acute model of infection with <scp>BALB<\/scp>\/c mice"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5090-5842","authenticated-orcid":false,"given":"Waldo Luis","family":"Garc\u00eda\u2010Jim\u00e9nez","sequence":"first","affiliation":[{"name":"Department of Pathology and Infectious Diseases School of Veterinary Medicine University of Surrey Guildford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5315-3882","authenticated-orcid":false,"given":"Francisco J.","family":"Salguero","sequence":"additional","affiliation":[{"name":"Department of Pathology and Infectious Diseases School of Veterinary Medicine University of Surrey Guildford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo V.","family":"D'Elia","sequence":"additional","affiliation":[{"name":"CBR Division Defence Science &amp; Technology Laboratory Salisbury UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/434692a"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002212"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1104.041051"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.01038.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172627"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00966-06"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002452"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.07.025"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.03.031"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00636-12"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2014.01.005"},{"key":"e_1_2_9_13_1","article-title":"Mechanisms of disease: host\u2010pathogen interactions between burkholderia species and lung epithelial cells","volume":"5","author":"David J.","year":"2015","journal-title":"Front. 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