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However, studies examining the effect of <jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content> due to high\u2010fat high\u2010fructose (HFHF) diet in factor <jats:styled-content style=\"fixed-case\">VIII<\/jats:styled-content>\u2010deficient (haemophilia A) animals or patients have not been reported previously. In this study, we investigated the individual role of factor <jats:styled-content style=\"fixed-case\">VIII<\/jats:styled-content> in the progression of diet\u2010induced <jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content> in the factor 8<jats:sup>\u2212\/\u2212<\/jats:sup> (F8<jats:sup>\u2212\/\u2212<\/jats:sup>) mouse model system and its consequences on the haemophilic status of the mice. The F8<jats:sup>\u2212\/\u2212<\/jats:sup> mice were fed with <jats:styled-content style=\"fixed-case\">HFHF<\/jats:styled-content> diet for 14\u00a0weeks. Physiological, biochemical, haematological, molecular, pathological, and immune histochemical analyses were performed to evaluate the effect of this diet. The F8<jats:sup>\u2212\/\u2212<\/jats:sup> mice developed hepatic steatosis after <jats:styled-content style=\"fixed-case\">14 weeks HFHF<\/jats:styled-content> diet and displayed lower energy metabolism, higher myeloid cell infiltration in the liver, decreased platelet count, upregulated <jats:italic>de novo<\/jats:italic> fatty acid synthesis, lipid accumulation, and collagen deposition. This study helps to understand the role of factor <jats:styled-content style=\"fixed-case\">VIII<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">NAFLD<\/jats:styled-content> pathogenesis and to analyse the severity and consequences of steatosis in haemophilic patients as compared to normal population. This study suggests that haemophilic animals (F8<jats:sup>\u2212\/\u2212<\/jats:sup> mice) are highly prone to hepatic steatosis and thrombocytopenia.<\/jats:p>","DOI":"10.1111\/iep.12264","type":"journal-article","created":{"date-parts":[[2018,4,15]],"date-time":"2018-04-15T20:01:53Z","timestamp":1523822513000},"page":"46-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of high\u2010fat high\u2010fructose diet treatment in factor <scp>VIII<\/scp> (coagulation factor)\u2010deficient mouse model"],"prefix":"10.1111","volume":"99","author":[{"given":"Alaknanda","family":"Mishra","sequence":"first","affiliation":[{"name":"National Institute of Immunology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shailendra","family":"Arindkar","sequence":"additional","affiliation":[{"name":"National Institute of Immunology New Delhi 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