{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T19:24:25Z","timestamp":1778527465489,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,6]],"date-time":"2019-01-06T00:00:00Z","timestamp":1546732800000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471334"],"award-info":[{"award-number":["81471334"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871002"],"award-info":[{"award-number":["81871002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["cstc2017jcjAX0211"],"award-info":[{"award-number":["cstc2017jcjAX0211"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to establish a robust model of diabetic myocardial hypertrophy in <jats:italic>Mus musculus castaneus<\/jats:italic> mice. Mice were fed a high\u2010fat diet for four\u00a0weeks and then given streptozotocin (STZ, 40\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup>\u00a0d<jats:sup>\u22121<\/jats:sup> for 5\u00a0days, intraperitoneally) and fasting blood glucose (FBG) levels were tested after seven\u00a0days. Mice with FBG levels above 11.1\u00a0mmol\/L were considered diabetic. Diabetic mice continued to have access to the high\u2010fat diet until cardiac hypertrophy developed. FBG and body weight (BW) were measured weekly. Myocardial hypertrophy was confirmed by left ventricle (LV) hypertrophy index (LVHI), LV\/BW, LV histopathological observation and atrial natriuretic factor (ANF) mRNA expression. Serum insulin and plasma haemoglobin A1c (HbA1c) levels, total cholesterol (TCH) and triglyceride (TG) were measured, and then an insulin resistance index (HOMA.IR) was calculated. The level of FBG in the model group remained above 11.1\u00a0mmol\/L, and the BW showed significant weight loss, compared with the control group (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01). The high levels of HbA1c, HOME.IR, TCH and TG, and the low level of insulin suggested that glucose metabolism was not balanced with insulin resistance; meanwhile, higher TCH and TG showed that dyslipidaemia had also developed. After the diabetic mice were kept on the high\u2010energy diet for another four \u00a0weeks, histopathological observation showed myocardial injuries, much more surface area and collagen fibres, higher LVHI and LV\/BW, and elevated expression of ANF mRNA (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01), suggesting that myocardial hypertrophy had appeared in <jats:italic>Mus musculus castaneus<\/jats:italic> mice under the current experimental conditions. Thus a robust model of diabetic myocardial hypertrophy was established four \u00a0weeks after confirmation of diabetes, which was induced by feeding a high\u2010fat diet for four weeks combined with a repeated low\u2010dose STZ exposure, in <jats:italic>Mus musculus castaneus<\/jats:italic> mice.<\/jats:p>","DOI":"10.1111\/iep.12296","type":"journal-article","created":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T19:30:28Z","timestamp":1546889428000},"page":"295-303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Establishment of a diabetic myocardial hypertrophy model in <i>Mus musculus castaneus<\/i> mouse"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6143-9055","authenticated-orcid":false,"given":"Jie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmacology Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumei","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical University  Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical University  Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingsong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.01.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00059.2007"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hfc.2012.06.007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199807270-00010"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1680049"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1258\/ebm.2010.010208"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.22063"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.45.6.749"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.817"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.5625\/lar.2016.32.3.160"},{"issue":"5","key":"e_1_2_8_12_1","first-page":"pp. 47.1\u20105.47.2","article-title":"Streptozotocin\u2010induced diabetic models in mice and rats","volume":"70","author":"Furman BL","year":"2015","journal-title":"Curr Protoc Pharmacol"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.27.5.1047"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/656795"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.10.027"},{"key":"e_1_2_8_16_1","first-page":"4099","article-title":"Effects of icariside II ameliorates diabetic cardiomyopathy in streptozotocin\u2010induced diabetic rats by activating Akt\/NOS\/NF\u2010\u03baB signaling","volume":"17","author":"Yang L","year":"2017","journal-title":"Mol Med\u2010Rep"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-017-0625-4"},{"key":"e_1_2_8_18_1","first-page":"469","article-title":"Cardio\u2010Protective role of Gingerol along with prominent anti\u2010diabetic cardiomyopathy action in a streptozotocin\u2010induced diabetes mellitus rat model","volume":"19","author":"Yu LY","year":"2017","journal-title":"Cell J"},{"key":"e_1_2_8_19_1","first-page":"356","article-title":"The streptozotocin\u2010induced diabetic nude mouse model: differences between animals from different sources","volume":"61","author":"Graham ML","year":"2011","journal-title":"Comp Med"},{"key":"e_1_2_8_20_1","first-page":"2121","article-title":"Validation of structural and functional lesions of diabetic retinopathy in mice","volume":"16","author":"Kern TS","year":"2010","journal-title":"Mol Vis"},{"key":"e_1_2_8_21_1","first-page":"20","article-title":"Comparison of streptozotocin\u2010induced C57B1\/6J and Kunming mouse type 1 diabetes model","volume":"1","author":"Zheng GY","year":"2016","journal-title":"Chin J Biochem Pharm"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2014.11.015"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083174"},{"key":"e_1_2_8_24_1","first-page":"30050","article-title":"Comorbidities and cardiometabolic disease: relationship with longitudinal changes in diastolic function","volume":"2213","author":"Nayor M","year":"2018","journal-title":"JACC Heart Fail"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.47.6.922"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18896-5"}],"container-title":["International Journal of Experimental Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiep.12296","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iep.12296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T04:01:51Z","timestamp":1694664111000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iep.12296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":25,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1111\/iep.12296"],"URL":"https:\/\/doi.org\/10.1111\/iep.12296","archive":["Portico"],"relation":{},"ISSN":["0959-9673","1365-2613"],"issn-type":[{"value":"0959-9673","type":"print"},{"value":"1365-2613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12]]},"assertion":[{"value":"2018-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}