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Rats were treated by intragastric gavage of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l4 or vehicle for 45\u00a0days. Blood plasma and different muscle types\u2014tibialis anterior (mostly white fibres), soleus (red muscle) and extensor digitorum longus (white muscle) \u2010 were analysed at the end of the study. Characteristic biomarkers of impaired hepatic function were found in the plasma of cirrhotic animals. The weights and protein contents of all muscles of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l4\u2010treated animals were lower when compared with controls. Increased concentrations of glutamine (<jats:styled-content style=\"fixed-case\">GLN<\/jats:styled-content>) and aromatic amino acids (phenylalanine and tyrosine) and decreased concentrations of branched\u2010chain amino acids (<jats:styled-content style=\"fixed-case\">BCAA<\/jats:styled-content>), glutamate (<jats:styled-content style=\"fixed-case\">GLU<\/jats:styled-content>), alanine and aspartate were found in plasma and muscles. In the soleus muscle, <jats:styled-content style=\"fixed-case\">GLN<\/jats:styled-content> increased more and <jats:styled-content style=\"fixed-case\">GLU<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BCAA<\/jats:styled-content> decreased less than in the extensor digitorum and tibialis muscles. Increased chymotrypsin\u2010like activity (indicating enhanced proteolysis) and decreased \u03b1\u2010ketoglutarate and <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> levels were found in muscles of cirrhotic animals. <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> concentration also decreased in blood plasma. It is concluded that a rat model of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>l4\u2010induced cirrhosis is a valid model for the investigation of hepatic cachexia that exhibits alterations in line with a theory of role of ammonia in pathogenesis of <jats:styled-content style=\"fixed-case\">BCAA<\/jats:styled-content> depletion, citric cycle and mitochondria dysfunction, and muscle wasting in cirrhotic subjects. The findings indicate more effective ammonia detoxification to <jats:styled-content style=\"fixed-case\">GLN<\/jats:styled-content> in red than in white muscles.<\/jats:p>","DOI":"10.1111\/iep.12299","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T05:14:15Z","timestamp":1547442855000},"page":"274-281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Muscle wasting and branched\u2010chain amino acid, alpha\u2010ketoglutarate, and <scp>ATP<\/scp> depletion in a rat model of liver cirrhosis"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-8881","authenticated-orcid":false,"given":"Milan","family":"Hole\u010dek","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Medicine in Hradec Kralove Charles University  Czech 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