{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:13:13Z","timestamp":1790057593359,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007807","name":"Japan Science Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2019,4]]},"abstract":"Summary<\/jats:title>\n \n Vitronectin (\n VN<\/jats:styled-content>\n ), an extracellular matrix protein, is a promising immune biomarker of non\u2010alcoholic steatohepatitis (\n NASH<\/jats:styled-content>\n ); however, its precise function remains unclear. This study investigated how\n VN<\/jats:styled-content>\n deficiency contributes to the development of\n NASH<\/jats:styled-content>\n . Towards this aim, wild\u2010type (\n WT<\/jats:styled-content>\n ) and\n VN<\/jats:styled-content>\n \u2212\/\u2212 mice were fed with a choline\u2010deficient, L\u2010amino acid\u2010defined, high\u2010fat diet (\n CDAHFD<\/jats:styled-content>\n ) for 6 and 10\u00a0weeks to induce\n NASH<\/jats:styled-content>\n , and the livers were isolated. In\n WT<\/jats:styled-content>\n mice fed with\n CDAHFD<\/jats:styled-content>\n for 6 and 10\u00a0weeks, the expression of\n Vn<\/jats:italic>\n mRNA<\/jats:styled-content>\n and protein was up\u2010regulated compared with that in mice fed with the\n MF<\/jats:styled-content>\n control diet, indicating that\n VN<\/jats:styled-content>\n is regulated in\n NASH<\/jats:styled-content>\n condition.\n VN<\/jats:styled-content>\n \u2212\/\u2212 mice showed decreased picrosirius red staining in the liver area and\n Col1a2<\/jats:italic>\n mRNA<\/jats:styled-content>\n expression levels, compared with\n WT<\/jats:styled-content>\n mice, indicating that the severity of hepatic fibrosis is attenuated in the\n CDAHFD<\/jats:styled-content>\n \u2010fed\n VN<\/jats:styled-content>\n \u2212\/\u2212 mice. In addition,\n VN<\/jats:styled-content>\n deficiency did not affect the area of lipid droplets in haematoxylin\u2010eosin staining and the\n mRNA<\/jats:styled-content>\n expression levels of fatty acid synthases,\n Srebp<\/jats:italic>\n ,\n Acc<\/jats:italic>\n and\n Fas<\/jats:italic>\n in the\n CDAHFD<\/jats:styled-content>\n \u2010fed mice. Moreover,\n VN<\/jats:styled-content>\n deficiency decreased the inflammation score and the\n mRNA<\/jats:styled-content>\n expression levels of\n Cd11b<\/jats:italic>\n and\n F4\/80<\/jats:italic>\n , macrophage markers, as well as\n Tnf\u2010\u03b1<\/jats:italic>\n and\n Il\u20101\u03b2<\/jats:italic>\n , inflammatory cytokines in the\n CDAHFD<\/jats:styled-content>\n \u2010fed mice. Furthermore,\n VN<\/jats:styled-content>\n deficiency decreased the protein and\n mRNA<\/jats:styled-content>\n expression levels of \u03b1\u2010smooth muscle actin in the\n CDAHFD<\/jats:styled-content>\n \u2010fed mice, suggesting that\n VN<\/jats:styled-content>\n deficiency inhibits the activation of hepatic stellate cells (\n HSC<\/jats:styled-content>\n s). Our findings indicate that\n VN<\/jats:styled-content>\n contributes to the development of fibrosis in the\n NASH<\/jats:styled-content>\n model mice via modulation of the inflammatory reaction and activation of HSCs.\n <\/jats:p>","DOI":"10.1111\/iep.12306","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T18:39:36Z","timestamp":1553020776000},"page":"72-82","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Vitronectin deficiency attenuates hepatic fibrosis in a non\u2010alcoholic steatohepatitis\u2010induced mouse model"],"prefix":"10.1111","volume":"100","author":[{"given":"Momoka","family":"Hayashida","sequence":"first","affiliation":[{"name":"Graduate School of Humanities and Sciences Ochanomizu University Tokyo Japan"},{"name":"Institute for Human Life Innovation Ochanomizu University Tokyo 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