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Blood plasma, liver, and soleus and extensor digitorum longus muscles were analysed after 10\u00a0days. The FF\u2010treated rats developed hepatomegaly associated with increased hepatic carnitine and ATP and AMP concentrations, decreased protein breakdown, and decreased concentrations of DNA and triglycerides. HFFD increased plasma ALT and AST activities. The weight and protein content of muscles in the HFFD group were lower compared with controls. In muscles of the LFFD group there were increased ATP and decreased AMP concentrations; in the HFFD group AMP was increased. In both FF\u2010treated groups  there were increased glycine, phenylalanine, and citrulline and decreased arginine and branched\u2010chain keto acids (BCKA) in blood plasma. After HFFD there were decreased levels of branched\u2010chain amino acids (BCAA; valine, leucine and isoleucine), methionine, and lysine and increased homocysteine. Decreased arginine and increased glycine concentrations were found in both muscles in FF\u2010treated animals; in HFFD\u2010treated animals lysine, methionine, and BCAA were decreased. We conclude that FF exerts protein\u2010anabolic effects on the liver and catabolic effects on muscles. HFFD causes signs of hepatotoxicity, impairs energy and protein balance in muscles, and decreases BCAA, methionine, and lysine. It is suggested that increased glycine and decreased lysine and methionine levels are due to activated carnitine synthesis; decreased BCAA and BCKA levels are due to increased BCAA oxidation.<\/jats:p>","DOI":"10.1111\/iep.12368","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T10:19:40Z","timestamp":1598955580000},"page":"171-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of low and high doses of fenofibrate on protein, amino acid, and energy metabolism in rat"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-8881","authenticated-orcid":false,"given":"Milan","family":"Hole\u010dek","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Medicine in Hradec Kr\u00e1lov\u00e9 Charles University Hradec Kr\u00e1lov\u00e9 Czech 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