{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T01:22:00Z","timestamp":1778808120170,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/14993\u20108"],"award-info":[{"award-number":["2019\/14993\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fibrosis is a common pathophysiological response of many tissues and organs subjected to chronic injury. Despite the diverse aetiology of keloid, lacaziosis and localized scleroderma, the process of fibrosis is present in the pathogenesis of all of these three entities beyond other individual clinical and histological distinct characteristics. Fibrosis was studied in 20 samples each of these three chronic cutaneous inflammatory diseases. An immunohistochemical study was carried out to explore the presence of \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) and vimentin cytoskeleton antigens, CD31, CD34, Ki67, p16; CD105, CD163, CD206 and FOXP3 antigens; and the central fibrotic cytokine TGF\u2010\u03b2. Higher expression of vimentin in comparison to \u03b1\u2010SMA in all three lesion types was found. CD31\u2010 and CD34\u2010positive blood vessel endothelial cells were observed throughout the reticular dermis. Ki67 expression was low and almost absent in scleroderma. p16\u2010positive levels were higher than ki67 and observed in reticular dermis of keloidal collagen in keloids, in collagen bundles in scleroderma and in the external layers of the granulomas in lacaziosis. The presence of \u03b1\u2010actin positive cells and rarely CD34 positive cells, observed primarily in keloids, may be related to higher p16 antigen expression, a measure of cell senescence. Low FOXP3 expression was observed in all lesion types. CD105\u2010positive cells were mainly found in perivascular tissue in close contact with the adventitia in keloids and scleroderma, while, in lacaziosis, these cells were chiefly observed in conjunction with collagen deposition in the external granuloma layer. We did not find high involvement of CD163 or CD206\u2010positive cells in the fibrotic process. TGF\u2010\u03b2 was notable only in keloid and lacaziosis lesions. In conclusion, we have suggested vimentin to be the main myofibroblast general marker of the fibrotic process in all three studied diseases, while endothelial\u2010to\u2010mesenchymal transition (EndoMT) and mesenchymal stem cells (MSCs) and M2 macrophages may not play an important role.<\/jats:p>","DOI":"10.1111\/iep.12456","type":"journal-article","created":{"date-parts":[[2022,10,2]],"date-time":"2022-10-02T06:45:12Z","timestamp":1664693112000},"page":"234-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Skin fibrosis associated with keloid, scleroderma and Jorge Lobo's disease (lacaziosis): An immuno\u2010histochemical study"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7556-0737","authenticated-orcid":false,"given":"Wagner Luiz","family":"Tafuri","sequence":"first","affiliation":[{"name":"Departamento de Patologia Geral Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"},{"name":"Faculdade de Medicina, Departamento de Patologia Universidade de S\u00e3o Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1431-6829","authenticated-orcid":false,"given":"Thaise Yumie","family":"Tomokane","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologia das Mol\u00e9stias Infecciosas \u2013 LIM50, Departamento de Patologia Faculdade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Maria Gon\u00e7alves","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciane","family":"Kanashiro\u2010Galo","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Departamento de Patologia Universidade de S\u00e3o Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David Miichael","family":"Mosser","sequence":"additional","affiliation":[{"name":"Department of Host\u2010Parasite Defense University of Maryland  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juarez Antonio Sim\u00f5es","family":"Quaresma","sequence":"additional","affiliation":[{"name":"Universidade Federal do Para, N\u00facleo de Medicina Tropical e Universidade Estadual do Para  Bel\u00e9m Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla","family":"Pagliari","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Departamento de Patologia Universidade de S\u00e3o Paulo  Sao Paulo Brazil"},{"name":"Instituto de Assist\u00eancia M\u00e9dica ao Servidor P\u00fablico Estadual e Programa de p\u00f3s\u2010gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade  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