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In this study, we investigated effects of Idebenone, a potent anti\u2010oxidant, on oxidative stress markers, the anti\u2010oxidant defence system, intracellular calcium concentrations and the inflammatory process in primary dystrophic muscle cells from <jats:italic>mdx<\/jats:italic> mice. Dystrophic muscle cells were treated with Idebenone (0.05\u2009\u03bcM) for 24\u2009h. The untreated <jats:italic>mdx<\/jats:italic> muscle cells were used as controls. The MTT assay showed that Idebenone did not have a cytotoxic effect on the dystrophic muscle cells. The Idebenone treatment was able to reduce the levels of oxidative stress markers, such as H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and 4\u2010HNE, as well as decreasing intracellular calcium influx in the dystrophic muscle cells. Regarding Idebenone effects on the anti\u2010oxidant defence system, an up\u2010regulation of catalase levels, glutathione reductase (GR), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity was observed in the dystrophic muscle cells. In addition, the Idebenone treatment was also associated with reduction in inflammatory molecules, such as nuclear factor kappa\u2010B (NF\u2010\u03baB) and tumour necrosis factor (TNF) in mdx muscle cells. These outcomes supported the use of Idebenone as a protective agent against oxidative stress and related signalling mechanisms involved in dystrophinopathies, such as DMD.<\/jats:p>","DOI":"10.1111\/iep.12463","type":"journal-article","created":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T09:40:08Z","timestamp":1671874808000},"page":"4-12","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Protection of dystrophic muscle cells using Idebenone correlates with the interplay between calcium, oxidative stress and inflammation"],"prefix":"10.1111","volume":"104","author":[{"given":"Amanda Harduim","family":"Valduga","sequence":"first","affiliation":[{"name":"Departamento de Biologia Estrutural e Funcional, Instituto de Biologia Universidade Estadual de Campinas (UNICAMP)  Campinas Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela 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