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There are limited treatments for it, and the pathogenesis is unclear. Therefore, there is an urgent need to explore the pathogenesis of liver fibrosis, and to try to identify new potential therapeutic targets. For this study we used the carbon tetrachloride abdominal injection induced liver fibrosis animal model in mice. Primary hepatic stellate cell isolation was performed by a density\u2010gradient separation method, and this was followed by immunofluorescence stain analyses. Signal pathway analysis was performed by dual\u2010luciferase reporter assay and western blotting. Our results showed that RUNX1 was upregulated in cirrhotic liver tissues compared with normal liver tissues. Besides, overexpression of RUNX1 caused more severe liver fibrosis lesions than control group under CCl<jats:sub>4<\/jats:sub>\u2010induced conditions. Moreover, \u03b1\u2010SMA expression in the RUNX1 overexpression group was significantly higher than in the control group. Interestingly, we found that RUNX1 could promote the activation of TGF\u2010\u03b2\/Smads in a dual\u2010luciferase reporter assay. Thus we demonstrated that RUNX1 could be considered as a new regulator of hepatic fibrosis by activating TGF\u2010\u03b2\/Smads signalling. Based on this, we concluded that RUNX1 may be developed as a new therapeutic target in the treatment of liver fibrosis in the future. In addition, this study also provides a new insight about the aetiology of liver fibrosis.<\/jats:p>","DOI":"10.1111\/iep.12474","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T06:38:49Z","timestamp":1681799929000},"page":"188-198","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<scp>RUNX1<\/scp> promotes liver fibrosis progression through regulating <scp>TGF<\/scp>\u2010\u03b2 signalling"],"prefix":"10.1111","volume":"104","author":[{"given":"Zhaoyang","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Shandong Provincial Hospital Shandong University  Jinan China"},{"name":"Department of Infectious Diseases Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan 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