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Here, we evaluated functional parameters of skeletal and cardiac striated muscle in two experimental models using the Morphometric Analysis of Muscle Fibre tool (MusMA). The collagen\u2010induced arthritis model was used to evaluate the function of skeletal striated muscle and the non\u2010alcoholic fatty liver disease model was used for cardiac striated muscle analysis. After euthanasia, we used haeamatoxylin and eosin stained sections of skeletal and cardiac muscle to perform muscle fibre segmentation and morphometric analysis. Morphometric analysis classified muscle fibres into six subpopulations: normal, regular hypertrophic, irregular hypertrophic, irregular, irregular atrophic and regular atrophic. The percentage of atrophic fibres was associated with lower walking speed (<jats:italic>p<\/jats:italic>\u2009=\u20090.009) and lower body weight (<jats:italic>p<\/jats:italic>\u2009=\u20090.026), respectively. Fibres categorized as normal were associated with maximum grip strength (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and higher march speed (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). In the evaluation of cardiac striated muscle fibres, the percentage of normal cardiomyocytes negatively correlated with cardiovascular risk markers such as the presence of abdominal adipose tissue (<jats:italic>p<\/jats:italic>\u2009=\u2009.003), miR\u201033a expression (<jats:italic>p<\/jats:italic>\u2009=\u2009.001) and the expression of miR\u2010126 (<jats:italic>p<\/jats:italic>\u2009=\u2009.042) Furthermore, the percentage of atrophic cardiomyocytes correlated significantly with the Castelli risk index II (<jats:italic>p<\/jats:italic>\u2009=\u2009.014). MusMA is a simple and objective tool that allows the screening of striated muscle fibre morphometry, which can complement the diagnosis of muscle diseases while providing functional and prognostic information in basic and clinical research.<\/jats:p>","DOI":"10.1111\/iep.12504","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T12:54:47Z","timestamp":1715259287000},"page":"100-113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Muscle fibre morphometric analysis (<scp>MusMA<\/scp>) correlates with muscle function and cardiovascular risk prognosis"],"prefix":"10.1111","volume":"105","author":[{"given":"Larisse","family":"Longo","sequence":"first","affiliation":[{"name":"Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental 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