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GSE17351 and GSE20347 data sets from the Gene Expression Omnibus (GEO) database were employed to screen differentially expressed genes (DEGs). Reverse transcription quantitative PCR (RT\u2010qPCR) was used to examine hub gene expression. ECA\u2010109 and TE\u201012 cells were transfected using the pcDNA3.1 expression vector encoding <jats:italic>GABRP<\/jats:italic>. The cell counting kit\u20108 (CCK\u20108), cell scratch and Transwell assays were performed to assess the effect of <jats:italic>GABRP<\/jats:italic> on EC cell proliferation, migration and invasion. Epithelial\u2010mesenchymal transition (EMT)\u2010associated protein levels were measured by Western blotting. Subsequently, <jats:italic>CFTR<\/jats:italic> was knocked down to verify whether <jats:italic>GABRP<\/jats:italic> affected biological events in EC cells by targeting <jats:italic>CFTR<\/jats:italic>. Seven hub genes were identified, including <jats:italic>GABRP<\/jats:italic>, <jats:italic>FLG<\/jats:italic>, <jats:italic>ENAH<\/jats:italic>, <jats:italic>KLF4<\/jats:italic>, <jats:italic>CD24<\/jats:italic>, <jats:italic>ABLIM3<\/jats:italic> and <jats:italic>ABLIM1<\/jats:italic>, which all could be used as diagnostic biomarkers for EC. The RT\u2010qPCR results indicated that the expression levels of <jats:italic>GABRP<\/jats:italic>, <jats:italic>FLG<\/jats:italic>, <jats:italic>KLF4<\/jats:italic>, <jats:italic>CD24<\/jats:italic>, <jats:italic>ABLIM3<\/jats:italic> and <jats:italic>ABLIM1<\/jats:italic> were downregulated, whereas the expression level of <jats:italic>ENAH<\/jats:italic> was upregulated. In\u00a0vitro functional assays demonstrated that <jats:italic>GABRP<\/jats:italic> overexpression suppressed the proliferation, migration, invasion and EMT of EC cells. Mechanistically, <jats:italic>GABRP<\/jats:italic> promoted the expression of <jats:italic>CFTR<\/jats:italic>, and <jats:italic>CFTR<\/jats:italic> knockdown significantly counteracted the influence of <jats:italic>GABRP<\/jats:italic> overexpression on biological events in EC cells. Overexpression of <jats:italic>GABRP<\/jats:italic> inhibited EC progression by increasing <jats:italic>CFTR<\/jats:italic> expression, which might be a new target for EC treatment.<\/jats:p>","DOI":"10.1111\/iep.12513","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T09:50:34Z","timestamp":1720691434000},"page":"118-132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>GABRP<\/i> inhibits the progression of oesophageal cancer by regulating <i>CFTR<\/i>: Integrating bioinformatics analysis and experimental validation"],"prefix":"10.1111","volume":"105","author":[{"given":"Jingzhi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology The Affiliated Ganzhou Hospital of Nanchang University  Ganzhou City 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