{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T17:28:18Z","timestamp":1776274098925,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T00:00:00Z","timestamp":1745971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302831\/2013\u20104"],"award-info":[{"award-number":["302831\/2013\u20104"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303320\/2013\u20103"],"award-info":[{"award-number":["303320\/2013\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303471\/2022\u20100"],"award-info":[{"award-number":["303471\/2022\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["2012\/13577\u20101"],"award-info":[{"award-number":["2012\/13577\u20101"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["2012\/15492\u20103"],"award-info":[{"award-number":["2012\/15492\u20103"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["04\/15526\u20109"],"award-info":[{"award-number":["04\/15526\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["08\/58491\u20101"],"award-info":[{"award-number":["08\/58491\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["11\/51697\u20106"],"award-info":[{"award-number":["11\/51697\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/04782\u20106"],"award-info":[{"award-number":["2014\/04782\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["17\/24051\u20104"],"award-info":[{"award-number":["17\/24051\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exercise has an important impact on skeletal muscle quality, emerging as an adjuvant therapy to ameliorate muscle inflammation and fibrosis in Duchenne muscular dystrophy (DMD). The aim of the present study was to investigate the benefit of exercise alone or in association with corticoid and omega\u20103 therapy in the middle aged mdx mouse model of DMD, Mdx mice (12\u2009months of age) performed treadmill exercise (12.4\u2009m\/min, for 15\u2009min, twice a week) for 4\u2009weeks. Exercised mdx received deflazacort (1.2\u2009mg\/kg; gavage) alone or combined with omega\u20103 (300\u2009mg\/kg; gavage). Sedentary mdx, C57BL\/10 and exercised mdx received mineral oil and served as control. At the endpoint (14\u2009months of age), muscle function, respiratory function, electrocardiography (ECG), blood markers of myonecrosis (creatine kinase, CK; alanine aminotransferase, ALT; and aspartate aminotransferase, AST), muscle biomarkers of inflammation and fibrosis (western blot), area of fibrosis and histopathology of tibialis anterior, biceps brachii and diaphragm muscles were evaluated. Exercise and exercise\u2010associated therapies improved behavioural activity (open field), muscle function (grip strength, four limb hanging test, hanging wire test and rotarod), VO<jats:sub>2<\/jats:sub> consumption, VCO<jats:sub>2<\/jats:sub> production and ECG. Functional benefits correlated with reduced myonecrosis (decreased CK, ALT, AST) and fibrosis. The muscles studied showed a reduction in inflammation biomarkers (NF\u2010\u03baB, IL\u20106 and TNF\u2010\u03b1) and fibrosis (TGF\u2010\u03b2 and fibronectin) and an increase in calsequestrin (calcium buffering protein) and PGC1\u2010\u03b1 (muscle integrity marker). In conclusion, exercise alone or associated with corticosteroid\/omega\u20103 therapy benefited limb, diaphragm and cardiac dystrophic muscles in middle\u2010aged mdx mice.<\/jats:p>","DOI":"10.1111\/iep.70000","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T05:29:29Z","timestamp":1746077369000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exercise combined with corticoid\/omega\u20103 therapy positively affected skeletal and cardiac muscles in middle aged <i>mdx<\/i> mice"],"prefix":"10.1111","volume":"106","author":[{"given":"Paula Andrea Saenz","family":"Suarez","sequence":"first","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Maciel","family":"Junior","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samara Cama\u00e7ari","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto","family":"Santo Neto","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9863-0761","authenticated-orcid":false,"given":"Elaine","family":"Minatel","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Julia","family":"Marques","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Myology","author":"Engel AG","year":"1994"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2017.08.007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-022-01272-x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.12965\/jer.1836408.204"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-022-03562-8"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42203-z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.4.1189"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012369454-6\/50074-1"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2012.11.013"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2016.01.015"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/ene.15184"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24563"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.041343"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2011.07.008"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21544"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2020.112609"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.24184"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6484610"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2018.10.008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00190.2011"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.947749"}],"container-title":["International Journal of Experimental Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iep.70000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T09:05:33Z","timestamp":1747645533000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iep.70000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,30]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/iep.70000"],"URL":"https:\/\/doi.org\/10.1111\/iep.70000","archive":["Portico"],"relation":{},"ISSN":["0959-9673","1365-2613"],"issn-type":[{"value":"0959-9673","type":"print"},{"value":"1365-2613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,30]]},"assertion":[{"value":"2024-06-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70000"}}