{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:34:28Z","timestamp":1753878868114,"version":"3.41.2"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T00:00:00Z","timestamp":1616112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001707","name":"Union for International Cancer Control","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001707","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Clinical Practice"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To describe histological features and pattern of expression of selected markers including epithelial growth factor receptor (EGFR), mutant p53 and mutant isocitrate dehydrogenase\u20101 (IDH\u20101R132H) among astrocytic neoplasms at the University College Hospital, Ibadan, Nigeria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>A retrospective cross\u2010sectional study involving histologically diagnosed Central Nervous System (CNS) neoplasms between January 2004 and December 2015. Haematoxylin and Eosin Slides of 81 cases of astrocytomas were retrieved, re\u2010cut and reviewed. Ethical clearance was obtained from the ethical board of the hospital. Immunohistochemistry using the Biotin\u2010Streptavidin system was performed with IDH\u20101 R132H, p53 and EGFR mouse monoclonal antibodies (MOABs) specific against all the cases of astrocytomas under review. All cases were graded and classified using the World Health Organisation (WHO) Classification of Central Nervous System tumours (2016). Membranous and cytoplasmic staining of EGFR and IDH\u20101R132H mouse monoclonal antibodies, respectively, were regarded as positive while nuclear staining of p53 mouse monoclonal antibody was regarded as positive. The data obtained were analysed with the level of statistical significance set at <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Males constituted a majority of cases, 50 (61.7%). Male\u2010Female ratio was 1.6:1. Mean age was 30.6\u00a0years. Tumours were of a higher WHO grade with increasing age, albeit glioblastoma cases tended to present at younger ages. The higher WHO grades were more likely to be located supratentorially. Glioblastomas accounted for most of the diagnosis 39 (48.1%), followed by pilocytic astrocytomas at 23 (28.4%). There was a low positive cytoplasmic expression of IDH\u20101 with only three (3.7%) being positive, eight (9.9%) showed a positive nuclear expression for mutant p53 while 17 (21%) showed membranous positivity for EGFR expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There are similar epidemiological trends between our cohort of patients and as described in most instances worldwide. Optimal stratification for astrocytomas can be achieved using a combination of IDH\u20101\/EGFR immunohistochemistry.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijcp.14094","type":"journal-article","created":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T07:22:34Z","timestamp":1614064954000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunohistochemical characterisation and histopathology of astrocytic neoplasms at a tertiary Nigerian hospital"],"prefix":"10.1155","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7563-8533","authenticated-orcid":false,"given":"Michael","family":"Nweke","sequence":"first","affiliation":[{"name":"Department of Pathology University College Hospital Ibadan Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Ogun","sequence":"additional","affiliation":[{"name":"Department of Pathology University College Hospital Ibadan Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amos","family":"Adeleye","sequence":"additional","affiliation":[{"name":"Department of Neurological Surgery University College Hospital Ibadan Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clement A.","family":"Okolo","sequence":"additional","affiliation":[{"name":"Department of Pathology University College Hospital Ibadan Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adekunle","family":"Adesina","sequence":"additional","affiliation":[{"name":"Department of Pathology Molecular Neuropathology Laboratory Texas Children\u2019s Hospital Baylor College of Medicine Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-007-0243-4"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1545-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808710"},{"issue":"3","key":"e_1_2_11_5_1","first-page":"611","article-title":"p53 mutation and protein alteration in 50 gliomas. 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