{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:09:34Z","timestamp":1792156174179,"version":"4.4.0"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,2,14]],"date-time":"2014-02-14T00:00:00Z","timestamp":1392336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The exact etiology of vitiligo remains obscure. Studies have indicated a role for cellular immunity in the pathogenesis of vitiligo. The aim of this study is to assess tissue <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxP3<jats:sup>+<\/jats:sup> natural regulatory <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cells (Tregs), as well as <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u2010cells in active vs. stable nonsegmental vitiligo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Immunohistochemical double\u2010staining for expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cells with immunostaining for expression of <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxP3 in lesional, marginal, and nonlesional skin of nonsegmental vitiligo was used to evaluate the abundance of Tregs among <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u2010cells in active and stable cases of vitiligo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significant increase in the number of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u2010cells and a highly significant reduction in the number of <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxP3\u2010expressing Tregs were detected in marginal skin in both stable and active vitiligo cases. <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>oxP3<jats:sup>+<\/jats:sup> cells were decreased in tissue of patients with vitiligo compared with healthy controls. The number of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u2010cells was increased in the epidermis and dermoepidermal junction (<jats:styled-content style=\"fixed-case\">DEJ<\/jats:styled-content>) in comparison with the number of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cells. Tregs were mostly present at the <jats:styled-content style=\"fixed-case\">DEJ<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The reduction in the number of FoxP3<jats:sup>+<\/jats:sup> cells in the marginal skin suggests that this is the site where regulatory activity is needed to suppress the activity of helper and cytotoxic T\u2010cells that are actively contributing to depigmentation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12160","type":"journal-article","created":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T04:58:35Z","timestamp":1392613115000},"page":"940-946","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Assessment of tissue FoxP3<sup>+<\/sup>, <scp>CD<\/scp>4<sup>+<\/sup> and <scp>CD<\/scp>8<sup>+<\/sup> T\u2010cells in active and stable nonsegmental 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