{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:41:56Z","timestamp":1770486116259,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T00:00:00Z","timestamp":1412035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Propylthiouracil (<jats:styled-content style=\"fixed-case\">PTU<\/jats:styled-content>), an anti\u2010thyroid thioureylene, has been shown to be effective in chronic plaque psoriasis. Involucrin is a precursor protein that is upregulated in psoriasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study evaluated the expression of involucrin in the epidermis of skin in psoriatic plaques before and after treatment with <jats:styled-content style=\"fixed-case\">PTU<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was an open\u2010label, prospective study in which 25 psoriasis patients underwent skin biopsies prior to treatment with oral <jats:styled-content style=\"fixed-case\">PTU<\/jats:styled-content> 100\u00a0mg three times per day for 12\u00a0weeks. Patients were assessed at 2, 6, and 12\u00a0weeks. Skin biopsies were repeated at the same sites at 12\u00a0weeks. Pre\u2010 and post\u2010treatment specimens were subjected to immunohistochemical staining and real\u2010time polymerase chain reaction for involucrin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean\u00a0\u00b1\u00a0standard deviation (<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>) scores on the <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>soriasis <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rea and <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>everity <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ndex reduced significantly from 17.86\u00a0\u00b1\u00a09.9 at baseline to 4.63\u00a0\u00b1\u00a04.1 at week\u00a012 (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001). Histomorphometric analysis revealed marked decreases in numbers of positively stained cells and intensity of staining. Staining became localized to the upper granular layers after therapy. Immunohistochemical scoring for involucrin reduced from a mean\u00a0\u00b1\u00a0<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content> of 9.00\u00a0\u00b1\u00a00.67 at baseline to 3.90\u00a0\u00b1\u00a00.88 at week\u00a012 (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In psoriasis, there is increased expression of involucrin, which leads to abnormal keratinocyte differentiation and hence to the formation of psoriatic plaques. The therapeutic effect of <jats:styled-content style=\"fixed-case\">PTU<\/jats:styled-content> in psoriasis may be attributable to the downregulation of involucrin. Larger trials should further elucidate the mechanism and therapeutic potential of <jats:styled-content style=\"fixed-case\">PTU<\/jats:styled-content> in psoriasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12565","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T09:23:54Z","timestamp":1412069034000},"page":"302-306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Downregulation of involucrin in psoriatic lesions following therapy with propylthiouracil, an anti\u2010thyroid thioureylene: immunohistochemistry and gene expression analysis"],"prefix":"10.1111","volume":"54","author":[{"given":"Pushpa","family":"Gnanaraj","sequence":"first","affiliation":[{"name":"Department of Dermatology Sri Ramaswamy Memorial (SRM) Medical College Hospital and Research Center Kattankulathur Kancheepuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haripriya","family":"Dayalan","sequence":"additional","affiliation":[{"name":"Department of Medical Research Sri Ramaswamy Memorial (SRM) Medical College Hospital and Research Center Kattankulathur Kancheepuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamilselvi","family":"Elango","sequence":"additional","affiliation":[{"name":"Department of Medical Research Sri Ramaswamy Memorial (SRM) Medical College Hospital and Research Center Kattankulathur Kancheepuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemamalini","family":"Malligarjunan","sequence":"additional","affiliation":[{"name":"Department of Medical Research Sri Ramaswamy Memorial (SRM) Medical College Hospital and Research Center Kattankulathur Kancheepuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijayashree","family":"Raghavan","sequence":"additional","affiliation":[{"name":"Department of Pathology Chettinad Hospital and Research Institute Kelambakkam Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramesh","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Pathology Chettinad Hospital and Research Institute Kelambakkam Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2003.12.003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1995.tb01598.x"},{"key":"e_1_2_6_4_1","first-page":"353","article-title":"Enhanced apoptosis in psoriatic plaques after treatment with propylthiouracil","volume":"17","author":"Elias AN","year":"2007","journal-title":"Eur J Dermatol"},{"key":"e_1_2_6_5_1","first-page":"1589","volume-title":"Textbook of Dermatology","author":"Camp RDR","year":"2011"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7525-4-35"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1993.tb05031.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.140S54001.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1619"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12665870"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00471.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12472288"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2010.06.006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004030050160"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.2.594"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(97)70320-7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009-0306"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4161\/derm.17027"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/10507250152484547"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1993.tb02845.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/0378-6323.86477"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/095466301317085354"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.12565","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.12565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T04:22:30Z","timestamp":1696306950000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.12565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,30]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1111\/ijd.12565"],"URL":"https:\/\/doi.org\/10.1111\/ijd.12565","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,30]]},"assertion":[{"value":"2014-09-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}