{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:22:24Z","timestamp":1762521744637},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Vitiligo is a depigmentary disease characterized by loss of melanocytes from the skin and mucous membranes. The pathomechanism of vitiligo is still obscure. Inducible nitric oxide synthase (i<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>) produces very large amounts of nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>). Promotor polymorphisms within i<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> gene have been reported to be associated with overproduction of <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>, which may induce melanocyte destruction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The current study aimed at investigating the possible association between i<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> gene polymorphism (\u2212954 G\/C and Ex 16+14 C\/T) and susceptibility to non\u2010segmental vitiligo in a cohort of <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>gyptians.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study was conducted on 200 participants: 100 patients with vitiligo and 100 aged matched healthy controls. Polymerase chain reaction using restriction fragment length polymorphism method (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>) was used to identify the genotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results showed that i<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> \u2212954\u00a0G\/C heteromutant genotype (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) was associated with increased risk of vitiligo (OR\u00a0=\u00a03.35, 95% CI\u00a0=\u00a01.77\u20136.33), and the risk increased when confined to females (OR\u00a0=\u00a07.4, 95% CI\u00a0=\u00a02.80\u201319.40). i<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> Ex 16 + 14 C\/T heteromutant genotype (<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>) conferred two folds increased risk of vitiligo (OR\u00a0=\u00a02.47, 95% CI\u00a0=\u00a01.39\u20134.37). Furthermore, the risk of vitiligo increased when the heteromutant genotype of i<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> \u2212954 G\/C (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) was co\u2010inherited with the wild genotype of <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content> Ex16+14 C\/T (CC) (OR\u00a0=\u00a023.2, 95% CI\u00a0=\u00a03.04\u2013177.21).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Inducible nitric oxide synthase \u2212954 G\/C and Ex 16+14 C\/T might be considered as genetic susceptibility markers for non\u2010segmental vitiligo among <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>gyptians.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12716","type":"journal-article","created":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T17:01:27Z","timestamp":1419872487000},"page":"675-679","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Inducible nitric oxide synthase promoter polymorphism: a molecular susceptibility marker for vitiligo in <scp>E<\/scp>gyptians"],"prefix":"10.1111","volume":"54","author":[{"given":"Amira A.","family":"Zayed","sequence":"first","affiliation":[{"name":"Faculty of Medicine Kasr Al Ainy University Hospital Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mervat M.","family":"Khorshied","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Kasr Al Ainy University Hospital Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marwa F.","family":"Hussein","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Kasr Al Ainy University Hospital Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470760116.ch2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2005.06.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.2007.00384.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-148X.2007.00429.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199312303292706"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-120-3-199402010-00009"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1089-8603(02)00127-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327110361760"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027077"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199712)183:4<469::AID-PATH931>3.0.CO;2-T"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8128228"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2010.02458.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2003.00032.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/315140"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i22.3278"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.12716","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.12716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T02:55:40Z","timestamp":1696301740000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.12716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,29]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1111\/ijd.12716"],"URL":"https:\/\/doi.org\/10.1111\/ijd.12716","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,29]]},"assertion":[{"value":"2014-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}