{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T11:03:43Z","timestamp":1749294223543,"version":"3.37.3"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,1,20]],"date-time":"2015-01-20T00:00:00Z","timestamp":1421712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National 973 Program of China","award":["2010CB529905"],"award-info":[{"award-number":["2010CB529905"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["NSFC81071861"],"award-info":[{"award-number":["NSFC81071861"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Condyloma acuminatum is one of the most commonly occurring sexually transmitted diseases. <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 is a small antimicrobial peptide that has been reported to have antiviral activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>Using the condyloma acuminatum tissue culture to resemble the situation more closely <jats:italic>in vivo<\/jats:italic>, we investigate the therapeutic effect of a recombinant plasmid encoding <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 gene in condyloma acuminatum tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Recombinant plasmid DNA carrying HNP1 c<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was constructed and identified. Then the recombinant plasmid was transfected into a condyloma acuminatum tissue fragment, and the <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 expression was determined on these tissue fragments by immunohistochemistry. <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> staining and flow cytometry techniques were used to examine cell apoptosis of condyloma acuminatum tissue. Relative real\u2010time polymerase chain reaction was used to validate antihuman papillomavirus therapeutics of the treatment groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transfected <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 gene was expressed mainly in the cytoplasmic granules of the condyloma acuminatum tissues. Positive apoptotic cells were observed in condyloma acuminatum tissues transfected with the <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 gene. In addition, the <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> expression was lower in the <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 treatment tissues as compared to their corresponding control tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results indicate that <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 can directly promote condyloma acuminatum cell apoptosis and play an antivirus role in the condyloma acuminatum tissue by limiting viral replication. These observations suggest a possible application for human <jats:styled-content style=\"fixed-case\">HNP<\/jats:styled-content>1 on condyloma acuminatum therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12725","type":"journal-article","created":{"date-parts":[[2015,1,20]],"date-time":"2015-01-20T09:11:08Z","timestamp":1421745068000},"page":"1205-1210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The therapeutic impact of <scp>HNP<\/scp>\u20101 in condyloma acuminatum"],"prefix":"10.1111","volume":"54","author":[{"given":"Shuyun","family":"Zhao","sequence":"first","affiliation":[{"name":"Biotherapy Laboratory of Gynecological Oncology Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Anatomy School of Preclinical and Forensic Medicine Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijuan","family":"Li","sequence":"additional","affiliation":[{"name":"Biotherapy Laboratory of Gynecological Oncology Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education West China Second University Hospital Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Yi","sequence":"additional","affiliation":[{"name":"Biotherapy Laboratory of Gynecological Oncology Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education West China Second University Hospital Sichuan University Chengdu 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