{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:51:45Z","timestamp":1790373105637,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T00:00:00Z","timestamp":1425427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective was to assess the relationship between enzyme\u2010linked immunoassay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) values of desmoglein (<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg) 1 and <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 antibodies and indirect immunofluorescence (<jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content>) values of anti\u2010epithelial antibodies with disease activity in patients with pemphigus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>In a retrospective study, we analyzed 353 serum samples taken from 35 patients with pemphigus vulgaris (<jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>) and nine with pemphigus foliaceus (<jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>) during the course of the disease. In each sample, we measured anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 and anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 antibodies by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. A receiver operating characteristics (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) curve was calculated to determine a cutoff value for anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 and anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 antibodies with optimal sensitivity and specificity. In 263 samples, we compared the <jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content> curves of anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 and anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 antibodies with the <jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content> curves of the <jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content> results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Activity of pemphigus was associated with a wide range of anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 and anti\u2010Dsg3 antibody values. Levels of anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 antibodies showed a better relationship with cutaneous activity of pemphigus than levels of <jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content> anti\u2010epithelial antibodies. The levels of <jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content> anti\u2010epithelial antibodies showed a relationship with activity of mucosas similar to the levels of anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Abnormal values of anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg antibodies are not always associated with disease activity. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> detects both pathogenic and nonpathogenic anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Therapeutic strategies should not be based exclusively on anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg antibody values. Anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg1 antibodies showed a closer relationship with skin activity than <jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content>, while anti\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>sg3 antibodies showed a relationship with mucosal activity similar to the <jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content> test.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12768","type":"journal-article","created":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T17:23:38Z","timestamp":1425489818000},"page":"1261-1268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Usefulness of specific anti\u2010desmoglein 1 and 3 enzyme\u2010linked immunoassay and indirect immunofluorescence in the evaluation of pemphigus activity"],"prefix":"10.1111","volume":"54","author":[{"given":"Maria A.","family":"Barnadas","sequence":"first","affiliation":[{"name":"Department of Dermatology Hospital Sta. Creu i St. Pau Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00aa Vict\u00f2ria","family":"Rubiales","sequence":"additional","affiliation":[{"name":"Department of Immunology Hospital Sta. Creu i St. Pau Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignasi","family":"Gich","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Hospital Sta. Creu i St. Pau Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Gelp\u00ed","sequence":"additional","affiliation":[{"name":"Department of Immunology Hospital Sta. Creu i St. Pau Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.26.4.268"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1979.04010040006002"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1001\/archderm.1980.01640270045011","article-title":"The correlation of disease activity and antibody titers in pemphigus","volume":"116","author":"Fizpatrick RE","year":"1980","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_5_1","first-page":"239","article-title":"Correlation of pemphigus vulgaris antibody titers by indirect immunofluorescence with activity of disease based on pemphigus area and activity score (PAAS)","volume":"46","author":"Mortavazi H","year":"2008","journal-title":"Acta Med Iran"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.74536"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12337524"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.4.2010"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.135.2.143"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.02752.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04132.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-0960.2005.00191.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2011.04428.x"},{"key":"e_1_2_6_14_1","first-page":"187864","article-title":"Detection of autoantibodies against recombinant desmoglein 1 and 3 molecules in patients with pemphigus vulgaris: correlation with disease extent at the time of diagnosis during follow\u2010up","author":"Belloni\u2010Fortina A","year":"2009","journal-title":"Clin Dev Immunol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2008.02715.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.9"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2007.02254.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/ced.12187"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1666"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2000.03538.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04312.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04838.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-0868"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2005.23714.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.000455"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.4.2170"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.10929.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.61"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.41"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2010.01069.x"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.12768","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.12768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T03:28:17Z","timestamp":1696217297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.12768"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,4]]},"references-count":29,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/ijd.12768"],"URL":"https:\/\/doi.org\/10.1111\/ijd.12768","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,4]]},"assertion":[{"value":"2013-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}