{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T22:10:45Z","timestamp":1759702245863},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T00:00:00Z","timestamp":1438041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"skin research center at Shahid Beheshti University of Medical Sciences"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Immunofluorescence antigen mapping (IFM), is a newly introduced technique for diagnosis and classification of epidermolysis bullosa (EB) disease. The precise level of skin cleavage can be determined using monoclonal antibodies to EB\u2010specific basement membrane zone protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To apply IFM technique in diagnosis and classification of EB and to identify utility and limitation of this method in our clinical setting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>IFM was done according to a described protocol by Pohla\u2010Gubo <jats:italic>et al<\/jats:italic>. Monoclonal antibodies used for antigen mapping were against cytokeratin 5, cytokeratin 14, \u03b16 integrin, <jats:italic>\u03b2<\/jats:italic>4 integrin, laminin 332, Collagen IV, and Collagen VII.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IFM was done for 95 referred patients, compromising 49 females and 46 males, aged 5 days to 45 years (mean = 9.5 years). Ninety cases were diagnosed with EB and classified as follows: EB simplex: (<jats:italic>n<\/jats:italic> = 13), junctional EB (<jats:italic>n<\/jats:italic> = 14), dystrophic EB (<jats:italic>n<\/jats:italic> = 62), and Kindler syndrome (<jats:italic>n<\/jats:italic> = 1). Diagnosis was not made in five cases as their specimens contained no blister. Confirmatory genetic analysis was done for five junctional cases from two families with clinical features of laryngo\u2010onycho\u2010cutaneous syndrome. Genetic molecular studies showed nonsense mutations in the last codon of exon 39 of the laminin \u03b13a (LAMA3) gene (p.Gln57X) and a donor splice site mutation in LAMA3 (IVS57+5G&gt;A) in the first and second family, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IFM technique is relatively simple to perform, and interpretation of the results is not sophisticated. The proportion of inconclusive results will be decreased if the specimens contain freshly induced blister.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12804","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T02:07:21Z","timestamp":1438135641000},"update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Using immunofluorescence (antigen) mapping in the diagnosis and classification of epidermolysis bullosa: a first report from Iran"],"prefix":"10.1111","volume":"54","author":[{"given":"Mohammadreza","family":"Barzegar","sequence":"first","affiliation":[{"name":"Skin Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Asadi\u2010kani","sequence":"additional","affiliation":[{"name":"Skin Research Center Shahid Beheshti University of Medical Sciences Tehran 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