{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:20:52Z","timestamp":1761513652343},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,6,12]],"date-time":"2015-06-12T00:00:00Z","timestamp":1434067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Leishmaniasis represents a polymorphous group of diseases caused by around 20 different species of <jats:italic>Leishmania<\/jats:italic> parasite. Increases in the number of cases of leishmaniasis reported as a consequence of the growth in travel and migration are of concern to epidemiologists and are diagnostically challenging in non\u2010endemic areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Molecular and histological analyses of a paraffin\u2010embedded skin biopsy were used in parallel to detect <jats:italic>Leishmania<\/jats:italic> parasites in a Cuban woman with suspicious lesions arriving in Cuba from Venezuela. Primers based on the 18S fragment of ribosomal ribonucleic acid (<jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>) and heat shock protein 70 genes (<jats:italic>hsp<\/jats:italic>70) were used for molecular detection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histological studies detected the presence of the parasite. A small fragment of <jats:italic>Leishmania <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was amplified by polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) targeting the 18S fragment using, for the first time, nucleic acid obtained from paraffin\u2010embedded tissue as a template. Amplification of a larger fragment from the <jats:italic>hsp<\/jats:italic>70 gene did not occur.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The detection of <jats:italic>Leishmania <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> from paraffin\u2010embedded tissue by means of 18S\u2010targeted <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> is a feasible approach to diagnosis. In combination with classical methods such as histology, the molecular detection of the parasite was demonstrated to be useful in confirming <jats:italic>Leishmania<\/jats:italic> infection in a traveler.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12915","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T23:49:15Z","timestamp":1434757755000},"page":"1175-1179","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular and histological tools to diagnose an imported case of American cutaneous leishmaniasis in Cuba"],"prefix":"10.1111","volume":"54","author":[{"given":"Ana M.","family":"Montalvo","sequence":"first","affiliation":[{"name":"Department of Parasitology Pedro Kour\u00ed Institute for Tropical Medicine Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaxsier","family":"De Armas","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Pedro Kour\u00ed Institute for Tropical Medicine Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Fraga","sequence":"additional","affiliation":[{"name":"Department of Parasitology Pedro Kour\u00ed Institute for Tropical Medicine Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orestes","family":"Blanco","sequence":"additional","affiliation":[{"name":"Hospital of the Pedro Kour\u00ed Institute for Tropical Medicine  Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinaldo","family":"Men\u00e9ndez","sequence":"additional","affiliation":[{"name":"Hospital of the Pedro Kour\u00ed Institute for Tropical Medicine  Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicente","family":"Montoto","sequence":"additional","affiliation":[{"name":"Provincial Center for Hygiene, Epidemiology and Microbiology Santiago de Cuba Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Cap\u00f3 de Paz","sequence":"additional","affiliation":[{"name":"Department of Pathology Pedro Kour\u00ed Institute for Tropical Medicine Havana Cuba"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1708-8305.2009.00341.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.03.001"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inhe.2009.12.005"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2011.07.001"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2013.18.30.20544"},{"key":"e_1_2_5_8_1","first-page":"108","article-title":"Diagn\u00f3stico de la leishmaniasis: de la observaci\u00f3n microsc\u00f3pica del par\u00e1sito a la detecci\u00f3n del ADN","volume":"64","author":"Montalvo AM","year":"2012","journal-title":"Rev Cubana Med Trop"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2008.02154.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/518041"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/524042"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002832"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1463-z"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.06.010"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.07.023"},{"key":"e_1_2_5_16_1","volume-title":"Laboratory Manual of Histochemistry","author":"Vacca L","year":"1985"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711410109"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000063150"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)10178-2"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000200013"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0365-05962011000500005"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2009.04099.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2011.01861.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90208-2"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/592509"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00259-09"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2011.09.013"},{"key":"e_1_2_5_28_1","volume-title":"Estudio y Distribuci\u00f3n de la Familia Phlebotominae. 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