{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T08:14:51Z","timestamp":1696407291010},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,10,24]],"date-time":"2015-10-24T00:00:00Z","timestamp":1445644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The diagnosis of dermatomyositis is sometimes difficult. We tried to evaluate the possibility that levels of <jats:italic>Homo sapiens<\/jats:italic> micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010214 (hsa\u2010miR\u2010214) in hair roots or hair shafts can be a useful marker of the disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A single hair root and five pieces of hair shafts were obtained from nine patients with dermatomyositis, 15 normal subjects, and 18 patients with scleroderma before treatment. <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were purified from the hair roots and hair shafts using commercially available kits. <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> was synthesized from the <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, and miR\u2010214 levels were measured with quantitative real\u2010time polymerase chain reaction. Receiver operating characteristic curve analysis was performed to evaluate the diagnostic performance of hair micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The levels of miR\u2010214 in hair shafts of patients with dermatomyositis were significantly higher than those of normal subjects and patients with scleroderma. By receiver operator curve analysis of hair shaft miR\u2010214 levels to distinguish patients with dermatomyositis from normal subjects, the area under the curve was 0.90. The duration between symptom onset and the first visit to the hospital was significantly shorter in patients with elevated hair shafts miR\u2010214 levels, suggesting that they have more severe subjective symptoms. On the other hand, we could not find significant differences in hair root miR\u2010214 levels among normal subjects and patients with dermatomyositis and scleroderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hair shaft miR\u2010214 levels are useful for diagnosis and evaluating the disease severity of dermatomyositis. Hair micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels may have potential to be a novel and less invasive biomarker.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12958","type":"journal-article","created":{"date-parts":[[2015,10,24]],"date-time":"2015-10-24T09:37:49Z","timestamp":1445679469000},"page":"786-790","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["micro<scp>RNA<\/scp> level is raised in the hair shafts of patients with dematomyositis in comparison with normal subjects and patients with scleroderma"],"prefix":"10.1111","volume":"55","author":[{"given":"Minami","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Dermatology and Plastic Surgery Faculty of Life Sciences Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Jinnin","sequence":"additional","affiliation":[{"name":"Department of 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