{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:25:34Z","timestamp":1760898334615},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,31]],"date-time":"2015-07-31T00:00:00Z","timestamp":1438300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"publisher","award":["NRF\u20102014R1A2A1A11051295","NRF\u20102009\u20100073055"],"award-info":[{"award-number":["NRF\u20102014R1A2A1A11051295","NRF\u20102009\u20100073055"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Keloids are marked by an overabundance of extracellular matrix. The antifibrotic effect of hepatocyte growth factor (<jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>) is achieved by increasing the expression of matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s) that drive extracellular matrix catabolism. As such, we cultivated an <jats:styled-content style=\"fixed-case\">RGD<\/jats:styled-content>\u2010modified <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>\u2010expressing adenovirus (<jats:styled-content style=\"fixed-case\">dE<\/jats:styled-content>1\u2010<jats:styled-content style=\"fixed-case\">RGD<\/jats:styled-content>\/lacZ\/<jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>) for introduction into keloid fibroblasts (<jats:styled-content style=\"fixed-case\">KF<\/jats:styled-content>s), looking at the subsequent impact on <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">KF<\/jats:styled-content>s infected with either test virus as experimental group (<jats:styled-content style=\"fixed-case\">dE<\/jats:styled-content>1\u2010<jats:styled-content style=\"fixed-case\">RGD<\/jats:styled-content>\/lacZ\/<jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>) or its counterpart (<jats:styled-content style=\"fixed-case\">dE<\/jats:styled-content>1\u2010<jats:styled-content style=\"fixed-case\">RGD<\/jats:styled-content>\/lacZ) as control group were examined for <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content> protein expression using an enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>). Collagen (types I and <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels were also determined by reverse transcriptase\u2013polymerase chain reaction, and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was used to monitor <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In <jats:styled-content style=\"fixed-case\">KF<\/jats:styled-content>s harboring the test virus, high levels of <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content> were induced at a multiplicity of infection ratio of 50 (3260.6\u00a0\u00b1\u00a0162.7\u00a0pg\/ml) after 72\u00a0hours of incubation. Furthermore, reverse transcriptase\u2013polymerase chain reaction and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> confirmed that <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein expression rose significantly in <jats:styled-content style=\"fixed-case\">KF<\/jats:styled-content>s after transduction by the test virus (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). However, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of collagen were unaffected by the experimental group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that an <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>\u2010expressing adenovirus may be therapeutic for keloids by increasing <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20101 expression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.12965","type":"journal-article","created":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T02:09:24Z","timestamp":1438394964000},"page":"356-361","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hepatocyte growth factor\u2010expressing adenovirus upregulates matrix metalloproteinase\u20101 expression in keloid fibroblasts"],"prefix":"10.1111","volume":"55","author":[{"given":"Yeo Reum","family":"Jeon","sequence":"first","affiliation":[{"name":"Institute for Human Tissue Restoration Department of Plastic &amp; Reconstructive Surgery Severance Hospital Yonsei University College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo Min","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Bioengineering College of Engineering Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il Kyu","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Bioengineering College of Engineering Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chae Ok","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Bioengineering College of Engineering Hanyang University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Kyun","family":"Rah","sequence":"additional","affiliation":[{"name":"Institute for Human Tissue Restoration Department of Plastic &amp; Reconstructive Surgery Severance Hospital Yonsei University College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dae Hyun","family":"Lew","sequence":"additional","affiliation":[{"name":"Institute for Human Tissue Restoration Department of Plastic &amp; Reconstructive Surgery Severance Hospital Yonsei University College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won Jai","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute for Human Tissue Restoration Department of Plastic &amp; Reconstructive Surgery Severance Hospital Yonsei University College of Medicine Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-199910000-00031"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1993.1132"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23601.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5001510"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977190902834077"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/5593"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(01)00245-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-010-1764-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.2001.6413"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2011.26.8.1081"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2004.09.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2003.08.242"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1402.065"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2007.02615.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2006.00168.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e31825dbf56"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.1626"},{"key":"e_1_2_7_19_1","first-page":"503","article-title":"Repeated intravenous injection of recombinant human hepatocyte growth factor ameliorates liver cirrhosis but causes albuminuria in rats","volume":"17","author":"Kusumoto K","year":"2006","journal-title":"Int J Mol Med"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2010.12.001"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22874"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000098686.72971.DB"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.09.006"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000070112.80711.3D"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2009.04.021"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8829"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/38410"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652311796150372"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2119\/2006-00031.Hirsch"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.843"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.12965","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.12965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T18:00:48Z","timestamp":1696356048000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.12965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,31]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1111\/ijd.12965"],"URL":"https:\/\/doi.org\/10.1111\/ijd.12965","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,31]]},"assertion":[{"value":"2014-10-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}