{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:46:35Z","timestamp":1790027195265,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,10,5]],"date-time":"2016-10-05T00:00:00Z","timestamp":1475625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Few studies have been published on the histopathology of cutaneous adverse drug reactions (<jats:styled-content style=\"fixed-case\">CADR<\/jats:styled-content>), and most of these lack information on skin allergological tests. The histopathology of drug reaction with eosinophilia and systemic symptoms (<jats:styled-content style=\"fixed-case\">DRESS<\/jats:styled-content>) is also seldom described. The purpose of our study was to examine six types of well\u2010documented<jats:styled-content style=\"fixed-case\">CADR<\/jats:styled-content>(maculopapular exanthema,<jats:styled-content style=\"fixed-case\">DRESS<\/jats:styled-content>, fixed drug eruption, Stevens\u2013Johnson syndrome, toxic epidermal necrolysis [<jats:styled-content style=\"fixed-case\">TEN<\/jats:styled-content>], and acute generalized exanthematous pustulosis) using histopathology and immunohistochemistry to evaluate the expression of granulysin, a key molecule in<jats:styled-content style=\"fixed-case\">TEN<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively included 106 skin biopsies performed in proven cases of<jats:styled-content style=\"fixed-case\">CADR<\/jats:styled-content>(by chronological investigation, single attributable drug, or\/and skin tests). All slides were reviewed, and microscopic changes were analyzed using a standardized form. Granulysin expression was studied by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In<jats:styled-content style=\"fixed-case\">DRESS<\/jats:styled-content>, we observed spongiosis, edema, and basal vacuolization, with rare necrotic keratinocytes and constant lymphocytic infiltrate in the superficial dermis. Eosinophils were often present, and pustules were found in 15% of cases. Necrotic keratinocytes are often absent in maculopapular exanthema. Granulysin was expressed in six types of<jats:styled-content style=\"fixed-case\">CADR<\/jats:styled-content>with a trend toward more intense expression in<jats:styled-content style=\"fixed-case\">DRESS<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">TEN<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We detailed further about the histopathology of<jats:styled-content style=\"fixed-case\">DRESS<\/jats:styled-content>. Granulysin expression was observed in all<jats:styled-content style=\"fixed-case\">CADR<\/jats:styled-content>with a marked overlap of expression pattern between the six types.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.13350","type":"journal-article","created":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T23:06:38Z","timestamp":1468623998000},"page":"1225-1233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Histopathological study of six types of adverse cutaneous drug reactions using granulysin expression"],"prefix":"10.1111","volume":"55","author":[{"given":"Marie","family":"Weinborn","sequence":"first","affiliation":[{"name":"Department of Dermatology Nancy University Hospital Brabois Hospital Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annick","family":"Barbaud","sequence":"additional","affiliation":[{"name":"Department of Dermatology Nancy University Hospital Brabois Hospital Vandoeuvre\u2010les\u2010Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Truchetet","sequence":"additional","affiliation":[{"name":"Department of Dermatology Metz\u2010Thionville Regional Hospital Thionville France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Beurey","sequence":"additional","affiliation":[{"name":"Pathology Center Emile Gall\u00e9 Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Germain","sequence":"additional","affiliation":[{"name":"Department of Clinical Epidemiology and Evaluation Nancy University Hospital Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Cribier","sequence":"additional","affiliation":[{"name":"Department of Dermatology Strasbourg University Hospital Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1991.01680080069004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0560.2001.028003113.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07704.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.09967.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2010.01612.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2010.0980"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2008.09.015"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2010.01611.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1884"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.12.022"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2001.450601.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1046\/j.0105-4538.2001.00001.x-i8","article-title":"General considerations for skin test procedures in the diagnosis of drug hypersensitivity","volume":"57","author":"Brockow K","year":"2002","journal-title":"Allergy"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12125"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12130"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08975.x"},{"key":"e_1_2_5_17_1","volume-title":"Weedon's Skin Pathology","author":"Weedon D","year":"2010"},{"key":"e_1_2_5_18_1","first-page":"2112","volume-title":"Cutaneous Adverse Reactions to Drugs","author":"McKee PH","year":"2005"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31820a285d"},{"key":"e_1_2_5_20_1","first-page":"37","article-title":"Acute generalized exanthematic pustuloses (four cases)","volume":"107","author":"Beylot C","year":"1980","journal-title":"Ann Dermatol Venereol"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2008.02585.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2009.08.050"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annder.2010.04.024"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12081"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12783"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1085-5629(96)80038-1"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2010.198"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12501"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02677.x"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2013.11.015"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.10921.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.07.010"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2012.01532.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.04.002"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.13350","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.13350","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T07:37:52Z","timestamp":1718696272000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.13350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,5]]},"references-count":33,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/ijd.13350"],"URL":"https:\/\/doi.org\/10.1111\/ijd.13350","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,5]]},"assertion":[{"value":"2015-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-10-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}