{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T15:49:17Z","timestamp":1771948157405,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T00:00:00Z","timestamp":1484006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guangdong Province Science and Technology Project","award":["2011B031800033"],"award-info":[{"award-number":["2011B031800033"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oxidative damage may lead to the dysfunction of melanocytes (<jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s) and is one of the causative mechanisms involved in the pathogenesis of vitiligo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study was designed to investigate the protective effects of the vitamin D3 analog tacalcitol on oxidative damage induced by hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) in human epidermal <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human epidermal <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s were cultured and identified by <jats:sc>l<\/jats:sc>\u2010<jats:styled-content style=\"fixed-case\">DOPA<\/jats:styled-content> staining and <jats:styled-content style=\"fixed-case\">HMB<\/jats:styled-content>\u201045 immunohistochemical staining. The model of oxidative damage induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> was established, and the cells were treated with tacalcitol. The viability of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s was determined using an <jats:styled-content style=\"fixed-case\">MTS<\/jats:styled-content> assay. Morphological changes in cell dendrites were observed by microscopy, and the rate of change of dendrites was calculated. The reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) level in <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s was determined using immunofluorescence microscopy. Superoxide dismutase (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>) activity and malondialdehyde (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>) levels in <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s were determined using the <jats:styled-content style=\"fixed-case\">WST<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">TBA<\/jats:styled-content> methods, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In comparison with the control group, the viability of <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> activity were significantly decreased in the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and significantly increased in the tacalcitol group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). In comparison with the control group, the rate of change of cell dendrites and levels of <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content> were significantly increased in the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and significantly decreased in the tacalcitol group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Tacalcitol can reduce oxidative damage induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in <jats:styled-content style=\"fixed-case\">MC<\/jats:styled-content>s by inhibiting intracellular <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> overproduction, increasing <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> activity, and decreasing the level of <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>, thereby reducing cell apoptosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.13407","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T10:07:50Z","timestamp":1484302070000},"page":"232-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Protective effects of tacalcitol against oxidative damage in human epidermal melanocytes"],"prefix":"10.1111","volume":"56","author":[{"given":"Qi\u2010Lin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology Guangzhou Red Cross Hospital Medical College Jinan University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Hua","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Guangzhou Red Cross Hospital Medical College Jinan University Guangzhou 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