{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T02:21:29Z","timestamp":1775096489443,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,1,12]],"date-time":"2017-01-12T00:00:00Z","timestamp":1484179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/25645\u20107"],"award-info":[{"award-number":["2014\/25645\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"Abstract<\/jats:title>\n \n Backgroud<\/jats:title>\n \n Atopic dermatitis (\n AD<\/jats:styled-content>\n ) is a chronic, inflammatory skin disease characterized by intense pruritus and xerosis. Dendritic cells (\n DC<\/jats:styled-content>\n ) play an essential role in tissue inflammation in atopic dermatitis (\n AD<\/jats:styled-content>\n ) skin, especially the inflammatory epidermal dendritic cells (\n IDEC<\/jats:styled-content>\n ), a particular subset of myeloid dendritic cells (\n mDC<\/jats:styled-content>\n ). The aim of the present study was to assess the phenotype and function of\n mDC<\/jats:styled-content>\n and circulating\n IDEC<\/jats:styled-content>\n \u2010like in peripheral blood mononuclear cells (\n PBMC<\/jats:styled-content>\n ) of adults with AD.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We selected 21\n AD<\/jats:styled-content>\n patients and 21 non\u2010\n AD<\/jats:styled-content>\n controls, age and gender matched. Expressions of Fc\n \u03b5<\/jats:italic>\n RI<\/jats:styled-content>\n ,\n CD<\/jats:styled-content>\n 36,\n TNF<\/jats:styled-content>\n ,\n IFN<\/jats:styled-content>\n \u2010\n \u03b3<\/jats:italic>\n , and\n IL<\/jats:styled-content>\n \u201010 in\n mDC<\/jats:styled-content>\n were analyzed by flow cytometry under various stimuli, such as staphylococcal enterotoxin B (\n SEB<\/jats:styled-content>\n ),\n TLR<\/jats:styled-content>\n 2 (Pam3\n CSK<\/jats:styled-content>\n 4),\n TLR<\/jats:styled-content>\n 4 (\n LPS<\/jats:styled-content>\n ), and\n TLR<\/jats:styled-content>\n 7\/8 (\n CL<\/jats:styled-content>\n 097) agonists.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The most prominent findings in\n AD<\/jats:styled-content>\n patients were: (i) enhanced frequency of\n IL<\/jats:styled-content>\n \u201010 under\n TLR<\/jats:styled-content>\n 4 (\n LPS<\/jats:styled-content>\n ), and decreased frequency of\n IFN<\/jats:styled-content>\n \u2010\n \u03b3<\/jats:italic>\n and\n TNF<\/jats:styled-content>\n under\n TLR<\/jats:styled-content>\n 2 (Pam3\n CSK<\/jats:styled-content>\n 4) and 7\/8 (\n CL<\/jats:styled-content>\n 097) stimulation in classic\n mDC<\/jats:styled-content>\n ; (ii) elevation of circulating\n IDEC<\/jats:styled-content>\n \u2010like frequency with\n TLR<\/jats:styled-content>\n 2 (Pam3\n CSK<\/jats:styled-content>\n 4) stimuli, augmented frequency of\n IFN<\/jats:styled-content>\n \u2010\n \u03b3<\/jats:italic>\n in nonstimulated condition\n ,<\/jats:italic>\n and of\n IL<\/jats:styled-content>\n \u201010 under\n TLR<\/jats:styled-content>\n 7\/8 (\n CL<\/jats:styled-content>\n 097) stimuli in\n IDEC<\/jats:styled-content>\n \u2010like population.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n In\n AD<\/jats:styled-content>\n individuals, classic\n mDC<\/jats:styled-content>\n showed an immunomodulatory profile, favoring tolerance in a combined action with\n IDEC<\/jats:styled-content>\n \u2010like, and inducing Th1 polarization. Our findings indicate a potential role of\n IDEC<\/jats:styled-content>\n \u2010like in the maintenance of inflammation in atopic dermatitis patients; moreover,\n IDEC<\/jats:styled-content>\n \u2010like may exert a regulatory impact on T cells of\n AD<\/jats:styled-content>\n individuals through\n IL<\/jats:styled-content>\n \u201010, often induced by agonist mimicking single stranded\n RNA<\/jats:styled-content>\n virus.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijd.13537","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T09:29:27Z","timestamp":1484299767000},"page":"630-635","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evidence of regulatory myeloid dendritic cells and circulating inflammatory epidermal dendritic cells\u2010like modulated by Toll\u2010like receptors 2 and 7\/8 in adults with atopic dermatitis"],"prefix":"10.1111","volume":"56","author":[{"given":"Vanessa G.","family":"dos Santos","sequence":"first","affiliation":[{"name":"Department of Dermatology Laboratory of Dermatology and Immunodeficiency (LIM\u201056) University of Sao Paulo School of Medicine Sao Paulo\u2010SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel L.","family":"Orfali","sequence":"additional","affiliation":[{"name":"Department of Dermatology Laboratory of Dermatology and Immunodeficiency (LIM\u201056) University of Sao Paulo School of Medicine Sao Paulo\u2010SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago","family":"de Oliveira Titz","sequence":"additional","affiliation":[{"name":"Department of Dermatology Laboratory of Dermatology and Immunodeficiency (LIM\u201056) University of Sao Paulo School of Medicine Sao Paulo\u2010SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto J.","family":"da Silva Duarte","sequence":"additional","affiliation":[{"name":"Department of Dermatology Laboratory of Dermatology and Immunodeficiency (LIM\u201056) University of Sao Paulo School of Medicine Sao Paulo\u2010SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria N.","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Dermatology Laboratory of Dermatology and Immunodeficiency (LIM\u201056) University of Sao Paulo School of Medicine Sao Paulo\u2010SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Aoki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.13-RAI-0564"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-015-0544-y"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.12.1563"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-14-29"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/231036"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.R-06-139"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2007.03.001"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12343596"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.225"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.03.006"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1670"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13214"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-016-0938-1"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-010-8224-0"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.11.019"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12333"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2007.094128"},{"issue":"92","key":"e_1_2_5_19_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.2340\/00015555924447","article-title":"Diagnostic features of atopic\u2010dermatitis","author":"Hanifin JM","year":"1980","journal-title":"Acta Derm\u2010Venereol"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2001.100102.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12324"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2012.03964.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00842.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.02.005"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxh186"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.01.062"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2000.00888.x"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.13537","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.13537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T16:35:37Z","timestamp":1695486937000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.13537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,12]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/ijd.13537"],"URL":"https:\/\/doi.org\/10.1111\/ijd.13537","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.727203639.793550877","asserted-by":"object"}]},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,12]]},"assertion":[{"value":"2016-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}