{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:10:30Z","timestamp":1787587830755,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The role of immunological factors in atopic dermatitis (<jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>) pathogenesis is well established. T\u2010helper (<jats:styled-content style=\"fixed-case\">TH<\/jats:styled-content>) cells are central in <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> pathogenesis. A relatively new subset of T cells, Th9 cells, was shown to be involved in the development of allergic asthma and allergic rhinitis, while its role in <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> is still to be investigated. This study aimed to measure gene expression levels of interleukin\u20109 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109) and <jats:styled-content style=\"fixed-case\">PU<\/jats:styled-content>.1, and to examine relationships with disease severity, serum IgE, and eruption types in <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study enrolled 30 <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> patients, 30 psoriasis patients, and 30 healthy subjects. The severity of <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> was assessed using the <jats:styled-content style=\"fixed-case\">SCORAD<\/jats:styled-content> index. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">PU<\/jats:styled-content>.1 expressions were measured by using real\u2010time quantitative polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RQ<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Serum IgE was measured by IgE (human) enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) Kit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109 and PU.1 gene expressions were significantly higher in <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> patients than in controls (<jats:italic>P<\/jats:italic>1 = 0.007, <jats:italic>P<\/jats:italic>2 &lt; 0.001, respectively). In the atopic dermatitis patients, expression of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109 and PU.1 were significantly positively correlated with <jats:styled-content style=\"fixed-case\">SCORAD<\/jats:styled-content> index (<jats:italic>P<\/jats:italic>1 = 0.004, <jats:italic>P<\/jats:italic>2 = 0.002) and clinically with erythema and edema scores. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">PU<\/jats:styled-content>.1 expressions were positively significantly correlated (<jats:italic>P<\/jats:italic> = 0.005) and positively correlated with serum IgE in the <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> group (<jats:italic>P<\/jats:italic>1 = 0.017, <jats:italic>P<\/jats:italic>2 = 0.023). No significant difference was noted between <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> patients with or without histories of other atopies regarding expression levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109 and PU.1 (<jats:italic>P<\/jats:italic>1 = 0.677, <jats:italic>P<\/jats:italic>2 = 0.135).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PU<\/jats:styled-content>.1 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20109 may play a role in <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> pathogenesis and relate to disease severity and clinical eruption types.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.13579","type":"journal-article","created":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T04:26:40Z","timestamp":1487824000000},"page":"534-539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Expression levels of transcription factor <scp>PU<\/scp>.1 and interleukin\u20109 in atopic dermatitis and their relation to disease severity and eruption types"],"prefix":"10.1111","volume":"56","author":[{"given":"Ashraf M.","family":"Hamza","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology &amp; Andrology Faculty of Medicine Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma S.","family":"Omar","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology &amp; Andrology Faculty of Medicine Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham A. 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