{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T11:51:35Z","timestamp":1785325895497,"version":"3.55.0"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,3]],"date-time":"2017-10-03T00:00:00Z","timestamp":1506988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Noncultured, extracted follicular outer root sheath suspension (<jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EHF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ORS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>) is a recently introduced technique for the treatment of stable vitiligo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the clinical efficacy of this technique and to determine the viability and cell composition of the suspension.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010five patients with stable vitiligo were included in this prospective study. Fifty follicles were extracted from occipital scalp and were incubated with trypsin\u2013ethylenediaminetetraacetic acid to separate outer root sheath cells. The cell suspension was filtered and centrifuged to obtain a cell pellet, which was resuspended and applied to the dermabraded recipient area. Cell viability of the suspension was assessed using trypan blue staining, and markers of keratinocyte stem cells (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200) and melanocytes (S100) were evaluated using flow cytometry and immunocytochemistry, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 6 months, the mean (\u00b1<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>) repigmentation was 52 \u00b1 25.1%, and &gt;75% repigmentation was seen in 8\/25 (32%) patients. Mean percentage cell viability of the suspension was 80 \u00b1 17.2% with a mean concentration of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 + and S100 + cells being 7.91 \u00b1 8.68% and 9.93 \u00b1 1.22% (n = 3), respectively. Recipient site infection was seen in 4 of 25 (16%) patients and a color mismatch in 11 of 25 (44%) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EHF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ORS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> is a useful minimally invasive therapy for vitiligo.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.13759","type":"journal-article","created":{"date-parts":[[2017,10,3]],"date-time":"2017-10-03T07:02:44Z","timestamp":1507014164000},"page":"245-249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Study of efficacy and safety of noncultured, extracted follicular outer root sheath cell suspension transplantation in the management of stable vitiligo"],"prefix":"10.1111","volume":"57","author":[{"given":"Pawan","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neetu","family":"Bhari","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj K.","family":"Tembhre","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujata","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Department of Stem cell facility All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudheer","family":"Arava","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod K.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3015-8602","authenticated-orcid":false,"given":"Somesh","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology All India Institute of Medical Sciences New Delhi 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