{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:08:19Z","timestamp":1787062099853,"version":"build-2736575974"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T00:00:00Z","timestamp":1509408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Syphilis remains a major challenge and a complex diagnosis. We aim to evaluate the role of polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) in\n Treponema pallidum<\/jats:italic>\n (Tp) detection in various types of biological samples in the diagnosis of early syphilis.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We conducted a cross\u2010sectional study including all attendees of the\n STI<\/jats:styled-content>\n clinic with clinical suspicion of early syphilis. One or more specimens for the detection of Tp by\n PCR<\/jats:styled-content>\n testing were collected.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The overall sensitivity of Tp\n PCR<\/jats:styled-content>\n test was 82.61% (95% CI: 68.6\u201392.2%). Tp\n PCR<\/jats:styled-content>\n test had sensitivity of 84.6% (95% CI: 54.6\u201398.1%) in primary syphilis cases and 81.8% (95% CI: 64.5\u201393%) in secondary syphilis cases.\n PCR<\/jats:styled-content>\n test performance was independent of\n HIV<\/jats:styled-content>\n status.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Tp\n PCR<\/jats:styled-content>\n test is a fast and reliable method for the detection of Tp in skin lesions of early syphilis, and it is a powerful tool in clinical settings.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijd.13823","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:36:40Z","timestamp":1509496600000},"page":"46-49","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Cross\u2010sectional study of\n Treponema pallidum<\/i>\n PCR in diagnosis of primary and secondary syphilis"],"prefix":"10.1111","volume":"57","author":[{"given":"Miguel","family":"Costa\u2010Silva","sequence":"first","affiliation":[{"name":"Dermatovenereology Department Centro Hospitalar de S\u00e3o Jo\u00e3o EPE Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department Centro Hospitalar do Baixo Vouga EPE Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Sobrinho\u2010Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Clinical Pathology Department Centro Hospitalar de S.Jo\u00e3o EPE Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Dermatovenereology Department Centro Hospitalar de S\u00e3o Jo\u00e3o EPE Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Lisboa","sequence":"additional","affiliation":[{"name":"Dermatovenereology Department Centro Hospitalar de S\u00e3o Jo\u00e3o EPE Porto Portugal"},{"name":"Microbiology Department Faculty of Medicine University of Porto Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,31]]},"reference":[{"key":"e_1_2_4_2_1","unstructured":"CharlesB.Pathophysiology transmission and natural history of syphilis. Literature review current through.http:\/\/www.uptodate.com\/contents\/syphilis-epidemiology-pathophysiology-and-clinical-manifestations-in-hiv-uninfected-patients. Accessed February 29 2016."},{"key":"e_1_2_4_3_1","volume-title":"Annual Epidemiological Report 2014 \u2013 Sexually Transmitted Infection Including HIV and Blood\u2010Born Viroses","author":"European Centre for Disease Prevention and Control (ECDC)"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050622"},{"key":"e_1_2_4_5_1","unstructured":"European Centre for Disease Prevention and Control.STD Surveillance Case Definitions.http:\/\/www.ecdc.europa.eu\/en\/activities\/surveillance\/sti\/Pages\/Case%20definition.aspx. Accessed April 19 2016."},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01578-06"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1370\/afm.215"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00702-11"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01619-15"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00720-09"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-353"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0b013e3181788186"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2008.11.901"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fijd.13823","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.13823","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T18:49:47Z","timestamp":1696013387000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.13823"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,31]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1111\/ijd.13823"],"URL":"https:\/\/doi.org\/10.1111\/ijd.13823","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,31]]},"assertion":[{"value":"2017-01-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}