{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T22:42:35Z","timestamp":1774737755602,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,11]],"date-time":"2017-11-11T00:00:00Z","timestamp":1510358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The objective of this study was to determine the presence of anti\u2010Dsg1 and Dsg3 antibodies in healthy subjects of the high Peruvian Amazon (Tuemal, Rodriguez de Mendoza province, department of Amazonas) to establish the theoretical presence of environmental factors or triggers in the area.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Cross\u2010sectional study. The study population included persons of any age or gender, clinically healthy, who were evaluated by a dermatologist to confirm the absence of blistering diseases. Blood samples were analyzed by indirect immunofluorescence (<jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content>), immunoprecipitation (<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>), anti\u2010Dsg1 IgM antibody (Ab) enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>), as well as anti\u2010Dsg1 and anti\u2010Dsg3 IgG Ab <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Participants included 21 healthy subjects comprised of 61.9% males and 38.1% females; 47.6% had a positive anti\u2010Dsg1 Ab <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> for total IgG (or any subclasses). <jats:styled-content style=\"fixed-case\">IIF<\/jats:styled-content> detected antibodies against intercellular spaces in one subject. Anti\u2010Dsg1 Ab <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> was mildly positive in 33.3% of the subjects. Anti\u2010Dsg1 IgG subclasses found positive were: IgG1 (19.0%), IgG2 (33.3%), and IgG3 (28.6%); none of the samples were positive for anti\u2010Dsg1 Ab IgM <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, and 23.8% of the subjects were positive for anti\u2010Dsg3 Ab <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. The age distribution was similar for subjects positive for anti\u2010Dsg1 and anti\u2010Dsg3 Ab <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, with higher frequencies found among the 20\u201329 and 40\u201349\u00a0year\u2010old age groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A fraction of healthy subjects of the high Peruvian Amazon developed anti\u2010Dsg1 and anti\u2010Dsg3 antibodies, demonstrating the possible presence of environmental factors for endemic pemphigus (<jats:styled-content style=\"fixed-case\">EP<\/jats:styled-content>) at a higher altitude than previously described.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.13824","type":"journal-article","created":{"date-parts":[[2017,11,11]],"date-time":"2017-11-11T13:02:42Z","timestamp":1510405362000},"page":"344-348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Antidesmoglein 1 and 3 antibodies in healthy subjects of a population in the Peruvian high amazon"],"prefix":"10.1111","volume":"57","author":[{"given":"Willy","family":"Ramos","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Cl\u00ednicas Universidad Nacional Mayor de San Marcos Lima Peru"},{"name":"Centro Nacional de Epidemiolog\u00eda Prevenci\u00f3n y Control de Enfermedades Ministerio de Salud Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Servicio de Dermatolog\u00eda Hospital Regional Docente Las Mercedes Chiclayo Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4725-6284","authenticated-orcid":false,"given":"Ericson L.","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Universidad de San Martin de Porres Lima Peru"},{"name":"Centro Nacional de Salud P\u00fablica Instituto Nacional de Salud Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose S.","family":"Lazarte","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Universidad de San Martin de Porres Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary C.","family":"Bohnett","sequence":"additional","affiliation":[{"name":"School of Medicine Oregon Health &amp; 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