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To improve understanding of <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content> in Kuwait, we report the experience of a local tertiary referral center over a 17.5\u00a0year period (January 2000\u2013June 2017) in establishing clinical and molecular diagnoses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Review of hospital records and diagnostic reports. Individual cases were diagnosed by combinations of clinical assessment, skin biopsy (immunohistochemistry and transmission electron microscopy), Sanger sequencing of <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content> genes, and whole exome sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifty\u2010four families with <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content> were registered with the clinic over this period, 41 of whom (84 patients) participated in diagnostic studies. Thirty\u2010seven of these 41 families had consanguineous marriages; 34 had recessive forms of <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content>, while only seven had dominant subtypes. Recurrent mutations were observed in epidermal dystonin, transglutaminase 5, and type <jats:styled-content style=\"fixed-case\">VII<\/jats:styled-content> collagen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The prevalence of <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content> in Kuwait is approximately three times that of internationally cited rates with an over\u2010representation of autosomal recessive variants. Establishing the molecular basis of <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content> in Kuwait with accurate diagnostic subtyping provides a basis for determining healthcare requirements and improving patient management of <jats:styled-content style=\"fixed-case\">EB<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.14099","type":"journal-article","created":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T14:25:50Z","timestamp":1531751150000},"page":"1058-1067","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Clinical subtypes and molecular basis of epidermolysis bullosa in Kuwait"],"prefix":"10.1111","volume":"57","author":[{"given":"Arti","family":"Nanda","sequence":"first","affiliation":[{"name":"As'ad Al\u2010Hamad Dermatology Center Salmiya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Liu","sequence":"additional","affiliation":[{"name":"National Diagnostic Epidermolysis Bullosa Laboratory, Viapath St. Thomas\u2019 Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hejab","family":"Al\u2010Ajmi","sequence":"additional","affiliation":[{"name":"As'ad Al\u2010Hamad Dermatology Center Salmiya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qasem A.","family":"Al\u2010Saleh","sequence":"additional","affiliation":[{"name":"As'ad Al\u2010Hamad Dermatology Center Salmiya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suad","family":"Al\u2010Fadhli","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences Faculty of Allied Health Sciences Kuwait University Safat Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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