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Understanding the differentially expressed genes between <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> states would be helpful for the early prevention and treatment of <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content>. Consequently, this study aimed to screen the key genes associated with the progression of <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The microarray dataset <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>45216 was downloaded from the Gene Expression Omnibus, which included 10 <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content> and 30 primary <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> skin tissue samples. Differentially expressed genes (<jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s) in <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> samples, compared to those in <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content>, were identified. Gene co\u2010expression relationships were investigated, followed by mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> prediction. The potential functions of the co\u2010expressed genes were predicted by gene ontology (<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>) and pathway enrichment analyses. In addition, the transcription factors and drug molecules, significantly related to the co\u2010expressed genes, were obtained.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 320 <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s were identified in the <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> group, relative to the <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content> group. Moreover, 96 <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s and 2,390 connecting edges were identified in the gene co\u2010expression network. An mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> regulatory network was constructed, including 96 <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s and 16 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s. In addition, three co\u2010expression network modules were obtained; <jats:italic><jats:styled-content style=\"fixed-case\">EIF<\/jats:styled-content>4<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>1<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">SNX<\/jats:styled-content>17<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">PRPF<\/jats:styled-content>4<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">NXT<\/jats:styled-content>1<\/jats:italic>, and <jats:italic><jats:styled-content style=\"fixed-case\">UBA<\/jats:styled-content>5<\/jats:italic> were significant nodes in the modules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">EIF<\/jats:styled-content>4<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>1<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">SNX<\/jats:styled-content>17<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">PRPF<\/jats:styled-content>4<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">NXT<\/jats:styled-content>1<\/jats:italic>, and <jats:italic><jats:styled-content style=\"fixed-case\">UBA<\/jats:styled-content>5<\/jats:italic> may be the pathogenic genes contributing to the development of <jats:styled-content style=\"fixed-case\">cSCC<\/jats:styled-content> from <jats:styled-content style=\"fixed-case\">AK<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.14131","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T05:50:03Z","timestamp":1534225803000},"page":"1208-1217","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Pathogenic genes related to the progression of actinic keratoses to cutaneous squamous cell 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