{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T16:27:59Z","timestamp":1788107279652,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T00:00:00Z","timestamp":1558396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> promoter methylation is usually an early stage in carcinogenesis process, including oral cancer. The purpose of this study was to investigate the association between T allele of specific single nucleotide polymorphism (<jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>) C&gt;T rs 16906252 and O16\u2010methylguanine\u2010DNA methyltransferase (MGMT) methylation as prospective biomarkers of malignant transformation in oral lichen planus (<jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>), a chronic autoimmune mucocutaneous disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This research is an observational, analytical case\u2013control study where a total of 85 subjects (43 control individuals and 42 <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> patients) participated. The samples (mouthwashes) from all volunteers were analyzed, and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> extraction was carried out. The genotyping of the rs 16906252 <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">MGMT<\/jats:styled-content> gene was performed by polymerase chain reaction\u2010restriction fragment length polymorphism (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>). Statistical analyses of Student <jats:italic>t<\/jats:italic> test and multiple logistic regressions were used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>C&gt;T genotype in the control and <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> groups was detected in 2.3% and 19.0%, respectively. The presence of this genotype was associated with methylation of the <jats:styled-content style=\"fixed-case\">MGMT<\/jats:styled-content> gene. In fact, taking into account age and gender, subjects with C&gt;T genotype were 10.5 (95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> 1.03\u2013106; <jats:italic>P\u00a0<\/jats:italic>=\u00a00.047) times more likely to methylate promoter region of the <jats:styled-content style=\"fixed-case\">MGMT<\/jats:styled-content> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings indicate that C&gt;T allele of rs 16906252, predictor of <jats:styled-content style=\"fixed-case\">MGMT<\/jats:styled-content> promoter methylation status, may be an important feature in the clinical prognosis of premalignant lesions of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>, although this finding requires further clinical and laboratory investigation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.14473","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T12:00:30Z","timestamp":1558440030000},"page":"1078-1082","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genotyping of the C&gt;T allele of rs16906252, predictor of O16\u2010methylguanine\u2010DNA methyltransferase (MGMT) promoter methylation status, in erosive atrophic lesions of oral lichen planus"],"prefix":"10.1111","volume":"58","author":[{"given":"Mariano","family":"S\u00e1nchez\u2010Siles","sequence":"first","affiliation":[{"name":"In private oral surgery and medicine practice Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Aliaga\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Oral and Maxillofacial Surgery Reina Sofia Hospital Murcia Spain"},{"name":"Department of Oral Surgery University of 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