{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:27:01Z","timestamp":1779114421654,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T00:00:00Z","timestamp":1578355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Vitiligo is mainly considered an autoimmune skin disease as the number of IL\u201017 producing Th17 cells, involved in the development of autoimmune and inflammatory pathologies, increased in vitiligo skin. T regulatory cells (Tregs) seem to be altered during the disease. Thus, there must be some upstream molecular factors that regulate the cellular response to apoptotic and inflammatory stimuli.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the expression of Th17\u2010 and Treg\u2010specific transcription factors in PBMCs and to evaluate the correlation between these transcription factors and cytokines in vitiligo patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated 30 active NSV patients for Th17\u2010 and Treg\u2010specific transcription factors ROR\u03b3t (retinoic acid\u2010related orphan receptor gamma t), FOXP3 (forkhead\/winged helix), HELIOS, EOS, and IRF4 (Interferon Regulatory Factor 4) as well as apoptotic marker NALP1 (NACHT\u2010leucine\u2010rich\u2010repeat protein 1) in PBMCs with RT\u2010qPCR. Immunostaining was done for transcription factors and cytokines on skin sections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mRNA level of FOXP3 was significantly lower in patients (0.76 fold, <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001), whereas ROR\u03b3t was slight but not\u00a0significantly increased (0.76 fold, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.06). Furthermore, NALP1 in lymphocytes was found to be increased in patients (0.69 fold, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). The immunostaining results revealed increased expression of ROR\u03b3, IL\u201017A, NALP1, and IL\u20101\u03b2 in vitiligo skin when compared to normal healthy skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reduced FOXP3\/ROR\u03b3t mRNA ratio suggests thriving of the Th17 cell population in PBMCs of vitiligo patients. Increased NALP1 levels indicate the existence of an apoptotic phenomenon which correlates with the increased expression of IL\u20101\u03b2 in vitiligo pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.14766","type":"journal-article","created":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T11:51:16Z","timestamp":1578397876000},"page":"474-481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Expression of Th17\u2010 and Treg\u2010specific transcription factors in vitiligo patients"],"prefix":"10.1111","volume":"59","author":[{"given":"Supriya","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Department of Dermatology Postgraduate Institute of Medical Education &amp; Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seema","family":"Rani","sequence":"additional","affiliation":[{"name":"Department of Zoology Punjab University Chandigarh 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