{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T07:13:40Z","timestamp":1779261220332,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,13]],"date-time":"2021-02-13T00:00:00Z","timestamp":1613174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Panniculitides are a heterogeneous group of inflammatory dermatoses involving the subcutaneous fatty tissue. Histologically, they are classified into septal and lobular panniculitis, according to the predominant localization of the inflammatory infiltrate. Neutrophils are frequently found in panniculitis, mainly at the early stages. Here, we investigated whether neutrophils contribute to various types of cutaneous panniculitis by releasing neutrophil extracellular traps (NETs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Formalin\u2010fixed paraffin\u2010embedded skin biopsies from 25 patients with panniculitis were included in the study. Our cohort was divided into <jats:italic>n<\/jats:italic>\u00a0=\u00a010 erythema nodosum (septal panniculitis) and <jats:italic>n<\/jats:italic>\u00a0=\u00a015 lobular panniculitis, including <jats:italic>n<\/jats:italic>\u00a0=\u00a07 lupus panniculitis, <jats:italic>n<\/jats:italic>\u00a0=\u00a01 pancreatic panniculitis, <jats:italic>n<\/jats:italic>\u00a0=\u00a01 Weber\u2010Christian disease, <jats:italic>n<\/jats:italic>\u00a0=\u00a01 deep fungal infection, <jats:italic>n<\/jats:italic>\u00a0=\u00a02 lipodermatosclerosis, and three cases did not have an identified etiology. The presence of neutrophils and NETs was assessed by double immunofluorescence using antibodies against elastase, a neutrophilic marker, and citrullinated histone 3, a marker of NETs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean percentages (\u00b1SEM) of elastase\u2010positive neutrophils showing NETs were 44%\u00a0\u00b1\u00a03% in erythema nodosum and 43%\u00a0\u00b1\u00a07% in lobular panniculitis. The difference was not statistically significant and reflects the implication of NETs not only in severe scarring lobular panniculitis but also in benign non\u2010scarring self\u2010remitting reactive inflammation such as erythema nodosum. In tissues, NETs were located in the interlobular septa in erythema nodosum and in the inflamed fat lobules in lobular panniculitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>NETs are massively present in septal and lobular subtypes of panniculitides, suggesting their involvement in tissue damage.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.15450","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T17:12:16Z","timestamp":1613322736000},"page":"724-729","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigating the presence of neutrophil extracellular traps in septal and lobular cutaneous panniculitides"],"prefix":"10.1111","volume":"60","author":[{"given":"R\u00e9mi","family":"Safi","sequence":"first","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"},{"name":"Department of Anatomy, Cell Biology and Physiological Science American University of Beirut Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georges","family":"El Hasbani","sequence":"additional","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara","family":"Bardawil","sequence":"additional","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ossama","family":"Abbas","sequence":"additional","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul\u2010Ghani","family":"Kibbi","sequence":"additional","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-0721","authenticated-orcid":false,"given":"Dany","family":"Nassar","sequence":"additional","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"},{"name":"Department of Anatomy, Cell Biology and Physiological Science American University of Beirut Beirut Lebanon"},{"name":"D\u00e9partement de Dermatologie Universit\u00e9 Paris Descartes H\u00f4pital Cochin Tarnier Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2001.114736"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2001.114735"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000985"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092385"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-018-0039-z"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2016.71"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3887"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00546"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aav5908"},{"key":"e_1_2_6_11_1","article-title":"Neutrophil Extracellular Traps are Present in 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