{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:21:01Z","timestamp":1784593261289,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T00:00:00Z","timestamp":1615680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The current study aims at evaluating the role of circulating or cell\u2010free long noncoding ribonucleic acid (lncRNA) growth arrest\u2010specific 5 (GAS5) in plaque psoriasis and at investigating its relationship with the presented clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This case\u2010control study was conducted on 180 subjects, divided into two main categories as follows: 90 cases with plaque psoriasis and 90 age\u2010 and sex\u2010matched healthy controls. Full history taking, thorough general examination, and full dermatological examination with determination of number and site of lesions were performed. Disease severity was assessed by Psoriasis Area Severity Index (PASI) score. Relative quantification of the expression level of cell\u2010free lncRNA (GAS5) was performed using real\u2010time polymerase chain reaction (RT\u2010PCR) technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was significant increase of GAS5 expression level in cases when compared with controls (<jats:italic>U<\/jats:italic>\u00a0=\u00a0719.0, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Indeed, there was significant positive correlation between GAS5 and PASI score (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.0.668, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Receiver operating characteristic (ROC) curve showed that GAS5 could identify patients from controls: GAS5 at a cut\u2010off value \u22650.31 provides a sensitivity of 95.56% and a specificity of 82.22%; at a cut\u2010off value \u22650.75, it can differentiate between mild and moderate cases, at a sensitivity of 77.78% and a specificity of 91.43%; at a cut\u2010off value \u22651.61, it can discriminate between moderate and severe cases, with a sensitivity of 71.43% and a specificity of 74.07%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>lncRNA GAS5 expression could be considered as a diagnostic marker of plaque psoriasis and indicator of its severity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.15494","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T03:25:21Z","timestamp":1615778721000},"page":"973-979","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Circulating long noncoding RNA growth arrest\u2010specific transcript 5 as a diagnostic marker and indicator of degree of severity in plaque psoriasis"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7126-8261","authenticated-orcid":false,"given":"Wafaa","family":"Ahmed Shehata","sequence":"first","affiliation":[{"name":"Dermatology, Andrology &amp; STDs Department Faculty of Medicine Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Maraee","sequence":"additional","affiliation":[{"name":"Dermatology, Andrology &amp; STDs Department Faculty of Medicine Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal","family":"Abd El Monem Ellaithy","sequence":"additional","affiliation":[{"name":"Medical Biochemistry &amp; Molecular Biology Faculty of Medicine Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nermin","family":"Tayel","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics &amp; Therapeutics Genetic Engineering and Biotechnology Research Institute Sadat City University Sadat Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmaa","family":"Abo\u2010Ghazala","sequence":"additional","affiliation":[{"name":"General Practitioner in Health Sector, Shebin El\u2010Kom Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally","family":"Mohammed El\u2010Hefnawy","sequence":"additional","affiliation":[{"name":"Medical Biochemistry &amp; Molecular Biology Faculty of Medicine Menoufia University Menoufia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.4006"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2020.05.008"},{"key":"e_1_2_7_4_1","first-page":"468","article-title":"Psoriasis and Metabolic Syndrome\u2013scientific evidence and therapeutic implications","volume":"7","author":"Voiculescu VM","year":"2014","journal-title":"J Med Life"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061475"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/jfmpc.jfmpc_689_19"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.147"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2521"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2011.04.001"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.03.004"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1138"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0390482"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.13425"},{"key":"e_1_2_7_14_1","first-page":"1680","article-title":"Real\u2010time P.C.R","volume":"50","author":"Dorak M","year":"2004","journal-title":"Clin Chem"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2008.02.039"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01749.x"},{"key":"e_1_2_7_17_1","first-page":"490","article-title":"Expression of Long Non\u2010coding RNA in Psoriasis","volume":"10","author":"El\u2010Rifaie AA","year":"2019","journal-title":"J Clin Exp Dermatol Res"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2638"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11661"},{"key":"e_1_2_7_20_1","first-page":"100665","article-title":"Dysregulation of cell\u2010free long non\u2010coding RNAs (NEAT2, CTC\u2010471J1.2 and lnc\u2010DC) in Egyptian systemic lupus and lupus nephritis patients. 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