{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:21:35Z","timestamp":1790050895907,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA215105"],"award-info":[{"award-number":["CA215105"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Approximately 85% of melanoma patients who undergo a sentinel lymph node biopsy (SLNB) are node\u2010negative. Melanoma incidence is highest in patients \u226565\u00a0years, but their SLNB positivity rate is lower than in younger patients. CP\u2010GEP, a model combining clinicopathologic and gene expression variables, identifies primary cutaneous melanoma (CM) patients who may safely forgo SLNB due to their low risk for nodal metastasis. Here, we validate CP\u2010GEP in a U.S. melanoma patient cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cohort of 208 adult patients with primary CM from the Mayo Clinic and West Virginia University was used. Patients were stratified according to their risk for nodal metastasis: CP\u2010GEP High Risk and CP\u2010GEP Low Risk. The main performance measures were SLNB reduction rate (RR) and negative predictive value (NPV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SLNB positivity rate for the entire cohort was 21%. Most patients had a T1b (34%) or T2a (31%) melanoma. In the T1\u2010T2 group (153 patients), CP\u2010GEP achieved an SLNB RR of 41.8% (95% CI: 33.9\u201050.1) at an NPV of 93.8% (95% CI: 84.8\u201098.3). Subgroup analysis showed similar performance in T1\u2010T2 patients \u226565\u00a0years of age (51 patients; SLNB positivity rate, 9.8%): SLNB RR of 43.1% (95% CI: 29.3\u201057.8) at an NPV of 95.5% (95% CI: 77.2\u201099.9).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We confirmed the potential of CP\u2010GEP to reduce negative SLNB in all relevant age groups. Our findings are especially relevant to patients \u226565\u00a0years, where surgery is often elective. CP\u2010GEP may guide SLNB decision\u2010making in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.15594","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T15:32:52Z","timestamp":1619710372000},"page":"851-856","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Validation of CP\u2010GEP (Merlin Assay) for predicting sentinel lymph node metastasis in primary cutaneous melanoma patients: A U.S. cohort study"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3827-339X","authenticated-orcid":false,"given":"Ahmed","family":"Yousaf","sequence":"first","affiliation":[{"name":"West Virginia University Morgantown WV USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9licia J.","family":"Tjien\u2010Fooh","sequence":"additional","affiliation":[{"name":"SkylineDx B.V. Rotterdam NL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Rentroia\u2010Pacheco","sequence":"additional","affiliation":[{"name":"SkylineDx B.V. Rotterdam NL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrica","family":"Quattrocchi","sequence":"additional","affiliation":[{"name":"Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajdin","family":"Kobic","sequence":"additional","affiliation":[{"name":"Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennie","family":"Tempel","sequence":"additional","affiliation":[{"name":"SkylineDx B.V. Rotterdam NL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kolodney","sequence":"additional","affiliation":[{"name":"West Virginia University Morgantown WV USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7462-8288","authenticated-orcid":false,"given":"Alexander","family":"Meves","sequence":"additional","affiliation":[{"name":"Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"e_1_2_5_2_1","volume-title":"National Cancer Institute: Surveillance, Epidemiology, and End Results Program","author":"Cancer Stat Facts: Melanoma of the Skin"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1310460"},{"key":"e_1_2_5_4_1","volume-title":"NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines). Melanoma: Cutaneous"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21409"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/SAP.0000000000001152"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2016.06.407"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocpiqo.2020.05.009"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2019.1643914"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2017.12.009"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-019-07204-2"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1200\/PO.19.00206"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.19499"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.14987"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181b07ffd"},{"key":"e_1_2_5_17_1","volume-title":"R: A language and environment for statistical computing","year":"2013"},{"key":"e_1_2_5_18_1","volume-title":"Gtsummary: Presentation\u2010Ready Data Summary and Analytic Result Tables","author":"Sjoberg DD","year":"2020"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2011.04.032"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.15594","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijd.15594","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.15594","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T07:58:16Z","timestamp":1693123096000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.15594"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,29]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1111\/ijd.15594"],"URL":"https:\/\/doi.org\/10.1111\/ijd.15594","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,29]]},"assertion":[{"value":"2020-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}