{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:14:10Z","timestamp":1792030450986,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T00:00:00Z","timestamp":1722729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2015\/18924\u20100"],"award-info":[{"award-number":["#2015\/18924\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rosacea is a chronic inflammatory disease, and doxycycline is a widely recommended treatment for it due to its anti\u2010inflammatory action. Oral isotretinoin reduces sebaceous gland activity and modulates toll\u2010like receptors, reducing inflammation. Our aim was to investigate the effect of these two drugs on the expression of cutaneous immunohistochemical biomarkers related to etiopathogenic factors involved in rosacea.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted a randomized, comparative, and evaluator\u2010blinded trial, including 40 participants with moderate and severe papulopustular and ocular rosacea. Participants were treated with doxycycline (DOXY) 100\u2009mg or isotretinoin (ISO) 0.3\u2009mg\/kg daily. Immunohistochemistry at baseline and after 4\u2009months was used to demonstrate the expression of the biomarker on the affected skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The following changes were detected: a reduction in the vessel count after using VEGF with DOXY (<jats:italic>P<\/jats:italic>\u2009=\u20090.010); a decrease in VEGF intensity with ISO (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and DOXY (<jats:italic>P<\/jats:italic>\u2009=\u20090.020); a reduction of nitric oxide synthase enzyme with both drugs in the inflammatory infiltrate (ISO <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001; DOXY <jats:italic>P<\/jats:italic>\u2009=\u20090.003); however, only with ISO was there a significant (<jats:italic>P<\/jats:italic>\u2009=\u20090.030) decrease at the level of the sebaceous glands, indicating a reduction of nitric oxide synthesis; a reduction of TRPV\u20101 expression at the level of the sebaceous glands was observed only with DOXY (<jats:italic>P<\/jats:italic>\u2009=\u20090.041); a decrease of cathelicidin LL37 expression, a key antimicrobial peptide in the etiopathogenesis of rosacea, was noted with both drugs, although at the level of sebaceous glands, only with DOXY (<jats:italic>P<\/jats:italic>\u2009=\u20090.007).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Oral isotretinoin and doxycycline have modified the expression of cutaneous biomarkers related to rosacea etiopathogenesis, demonstrating their role in controlling inflammatory and vascular processes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijd.17420","type":"journal-article","created":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T17:25:20Z","timestamp":1722792320000},"page":"546-551","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A comparative exploration of immunohistochemical markers in patients with papulopustular rosacea undergoing treatment with oral isotretinoin versus doxycycline"],"prefix":"10.1111","volume":"64","author":[{"given":"Fab\u00edola","family":"Picosse","sequence":"first","affiliation":[{"name":"Department of Dermatology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1090-177X","authenticated-orcid":false,"given":"Marco Alexandre","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline Sousa","family":"Costa","sequence":"additional","affiliation":[{"name":"Discipline of Dermatology, Department of Specialized Medicine Universidade Federal do Piau\u00ed  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milvia Maria Sim\u00f5es e Silva","family":"Enokihara","sequence":"additional","affiliation":[{"name":"Department of Pathology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Sanudo","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7190-8241","authenticated-orcid":false,"given":"Edil\u00e9ia","family":"Bagatin","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Escola Paulista de Medicina Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.17590"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2013.04.040"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.01.023"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15668"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000000888"},{"key":"e_1_2_8_7_1","first-page":"585","article-title":"The face and mind evaluation study: an examination of the efficacy of rosacea treatment using physician ratings and patients' self\u2010reported quality of life","volume":"4","author":"Fleischer A","year":"2005","journal-title":"J Drugs 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Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijd.17420","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T17:27:57Z","timestamp":1747675677000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijd.17420"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,4]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/ijd.17420"],"URL":"https:\/\/doi.org\/10.1111\/ijd.17420","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,4]]},"assertion":[{"value":"2023-12-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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