{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:55:36Z","timestamp":1790816136257,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,9]],"date-time":"2013-01-09T00:00:00Z","timestamp":1357689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Human leucocyte antigen (<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>) genes play an important role in the success of organ transplantation and are associated with autoimmune and infectious diseases. Current <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010based genotyping methods, including Sanger sequence\u2010based typing (<jats:styled-content style=\"fixed-case\">SSBT<\/jats:styled-content>), have identified a high degree of polymorphism. This level of polymorphism makes high\u2010resolution <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> genotyping challenging, resulting in ambiguous typing results due to an inability to resolve phase and\/or defining polymorphisms lying outside the region amplified. Next\u2010generation sequencing (<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>) may resolve the issue through the combination of clonal amplification, which provides phase information, and the ability to sequence larger regions of genes, including introns, without the additional effort or cost associated with current methods. The <jats:styled-content style=\"fixed-case\">NGS HLA<\/jats:styled-content> sequencing project of the 16<jats:styled-content style=\"fixed-case\">IHIW<\/jats:styled-content> aimed to discuss the different approaches to (i) template preparation including short\u2010 and long\u2010range <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> amplicons, exome capture and whole genome; (ii) sequencing platforms, including <jats:styled-content style=\"fixed-case\">GS<\/jats:styled-content> 454 <jats:styled-content style=\"fixed-case\">FLX<\/jats:styled-content>, Ion Torrent <jats:styled-content style=\"fixed-case\">PGM<\/jats:styled-content>, Illumina <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>eq\/<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>eq and <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>acific <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>iosciences <jats:styled-content style=\"fixed-case\">SMRT<\/jats:styled-content>; (iii) data analysis, specifically allele\u2010calling software. The pilot studies presented at the workshop demonstrated that although individual sequencers have very different performance characteristics, all produced sequence data suitable for the resolution of <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> genotyping ambiguities. The developments presented at this workshop clearly highlight the potential benefits of <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> laboratory.<\/jats:p>","DOI":"10.1111\/iji.12024","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T10:43:05Z","timestamp":1444732985000},"page":"72-76","source":"Crossref","is-referenced-by-count":74,"title":["16<sup>th<\/sup> IHIW : Review of HLA typing by NGS"],"prefix":"10.1111","volume":"40","author":[{"given":"D.","family":"De Santis","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology PathWest, Royal Perth Hospital Perth WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Dinauer","sequence":"additional","affiliation":[{"name":"Life Technologies Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Duke","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine University of Pennsylvania and The Children's Hospital of Philadelphia Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. A.","family":"Erlich","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Roche Molecular Systems Pleasanton CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. L.","family":"Holcomb","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Roche Molecular Systems Pleasanton CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Lind","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine University of Pennsylvania and The Children's Hospital of Philadelphia Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Mackiewicz","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine University of Pennsylvania and The Children's Hospital of Philadelphia Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Monos","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine University of Pennsylvania and The Children's Hospital of Philadelphia Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Moudgil","sequence":"additional","affiliation":[{"name":"Departments of Structural Biology, Microbiology and Immunology Stanford University School of Medicine Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Norman","sequence":"additional","affiliation":[{"name":"Departments of Structural Biology, Microbiology and Immunology Stanford University School of Medicine Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Parham","sequence":"additional","affiliation":[{"name":"Departments of Structural Biology, Microbiology and Immunology Stanford University School of Medicine Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Sasson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Perelman School of Medicine University of Pennsylvania and The Children's Hospital of Philadelphia Philadelphia PA USA"},{"name":"Bioinformatics Core The Children's Hospital of Philadelphia Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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N.","family":"Allcock","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology PathWest, Royal Perth Hospital Perth WA Australia"},{"name":"Lotterywest State Biomedical Facility Genomics, School of Pathology and Laboratory Medicine University of Western Australia Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,9]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2009.01345.x"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2007.01.014"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.2011.01040.x"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60915-5"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2012.01881.x"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1333"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2010.01606.x"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-06-097386"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2005.07.001"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07873-X"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2866"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2012.01941.x"}],"container-title":["International Journal of Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiji.12024","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.12024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T07:17:23Z","timestamp":1694675843000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iji.12024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,9]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/iji.12024"],"URL":"https:\/\/doi.org\/10.1111\/iji.12024","archive":["Portico"],"relation":{},"ISSN":["1744-3121","1744-313X"],"issn-type":[{"value":"1744-3121","type":"print"},{"value":"1744-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,9]]}}}