{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T05:10:46Z","timestamp":1696309846003},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,31]],"date-time":"2013-05-31T00:00:00Z","timestamp":1369958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Accurate human leucocyte antigen (<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>) typing results are essential in determining the degree of compatibility between donor and recipient in both solid organ (<jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content>) and hematopoietic stem cell (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>) transplantation. Current <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> typing methodologies can generate ambiguous results which may need resolving. This group\u2010specific sequencing approach allowed investigation into the presence of the low expressor <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*24:02:01:02L allele and the rare <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*02:64 allele in a <jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content> transplant recipient and a <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> transplant recipient, respectively. Locus\u2010specific amplification of <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A was performed. Exons 2 and 3 were sequenced in both directions followed by group\u2010specific sequencing to resolve ambiguities. Hemizygous sequence data of intron 2 generated from the <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*24 allele indicated the presence of the <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*24:02:01:01 allele. <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A*02:64 was identified by sequencing the allele in isolation over exons 2 and 3 and allowed confirmation of this allele sequence with the <jats:styled-content style=\"fixed-case\">IMGT<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> database (Accession number <jats:styled-content style=\"fixed-case\">AY<\/jats:styled-content>297166). This approach is cost efficient and can be modified to sequence alleles at other <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> loci. It has also been adapted to characterize the novel <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DQB<\/jats:styled-content>1*06:48 allele (Accession number <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>647646) as well as the non\u2010<jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> gene, <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>2B17, making it a useful tool to augment existing typing methodologies.<\/jats:p>","DOI":"10.1111\/iji.12070","type":"journal-article","created":{"date-parts":[[2013,5,31]],"date-time":"2013-05-31T15:27:34Z","timestamp":1370014054000},"page":"453-459","source":"Crossref","is-referenced-by-count":0,"title":["A group\u2010specific sequencing approach to investigate the presence of atypical human leucocyte antigen alleles"],"prefix":"10.1111","volume":"40","author":[{"given":"L.","family":"Foster","sequence":"first","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Tate","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Poulton","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,31]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Applied Biosystems. 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