{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T20:50:10Z","timestamp":1760820610777},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,6,11]],"date-time":"2014-06-11T00:00:00Z","timestamp":1402444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Summary<\/jats:title>Coeliac disease (CD<\/jats:styled-content>) is a highly prevalent autoimmune disorder that is triggered by the ingestion of wheat gluten and related proteins in genetically susceptible individuals. The CD<\/jats:styled-content> is associated with human leucocyte antigen (HLA<\/jats:styled-content>) genes particularly with HLA<\/jats:styled-content>\u2010DQ<\/jats:styled-content> alleles encoding HLA<\/jats:styled-content>\u2010DQ<\/jats:styled-content>2 and DQ<\/jats:styled-content>8 proteins. To define risk and severity alleles for CD<\/jats:styled-content>, a total of 120 definite CD<\/jats:styled-content> patients and 100 healthy controls were genotyped for HLA<\/jats:styled-content>\u2010DQB<\/jats:styled-content>1 gene. HLA<\/jats:styled-content>\u2010DQB<\/jats:styled-content>1 genotyping was performed in all patients and controls using PCR<\/jats:styled-content>\u2010SSP<\/jats:styled-content> technique, and to evaluate the clinical relevance of testing for HLA<\/jats:styled-content>\u2010DQB<\/jats:styled-content>1 and determining absolute risk of disease, prevalence\u2010corrected positive predictive value and prevalence\u2010corrected negative predictive value (PcPPV<\/jats:styled-content> and PcNPV<\/jats:styled-content>) were calculated. Our results for a first time show that DQB<\/jats:styled-content>1*02:00 and DQB<\/jats:styled-content>1*03:02 alleles and DQB<\/jats:styled-content>1*02:01\/03:02 genotype very significantly associated with increased risk of patients with CD<\/jats:styled-content>, and DQB<\/jats:styled-content>1*03:01,4 allele provides protection against CD<\/jats:styled-content> in Iranian patients. Furthermore, the PcPPV<\/jats:styled-content> for DQB<\/jats:styled-content>*02:01 and 03:02 alleles in CD<\/jats:styled-content> were 0.014 and 0.012, respectively, and the highest absolute risk presented by DQB<\/jats:styled-content>*0201\/0302 genotype (PcPPV<\/jats:styled-content>\u00a0=\u00a00.079) and 98% of patients with CD<\/jats:styled-content> carried DQB<\/jats:styled-content>1*02:01\/x or DQB<\/jats:styled-content>1*03:02\/x genotype. The results also clearly demonstrated that the DQB<\/jats:styled-content>1*02:01 allele significantly associated with severity of CD<\/jats:styled-content>, while DQB<\/jats:styled-content>1*03:02 allele associated with mild form of CD<\/jats:styled-content>. These results suggest that clinically suspected individuals for CD<\/jats:styled-content> and first\u2010degree relatives of patients with CD<\/jats:styled-content> to be screened for HLA<\/jats:styled-content>\u2010DQB<\/jats:styled-content>*0201 and DQB<\/jats:styled-content>*0302 alleles for possible early diagnosis and treatments.<\/jats:p>","DOI":"10.1111\/iji.12128","type":"journal-article","created":{"date-parts":[[2014,6,11]],"date-time":"2014-06-11T10:39:21Z","timestamp":1402483161000},"page":"312-317","source":"Crossref","is-referenced-by-count":13,"title":["The involvement of the HLA<\/scp>\u2010DQB<\/scp>1 alleles in the risk and the severity of Iranian coeliac disease patients"],"prefix":"10.1111","volume":"41","author":[{"given":"M.","family":"Zamani","sequence":"first","affiliation":[{"name":"Faculty of Medicine Department of Medical Genetics Tehran University of Medical Sciences Tehran Iran"},{"name":"Department of Neurogenetics Iranian Centre of Neurological Research Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Modares\u2010sadegi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Medical Genetics Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Shirvani","sequence":"additional","affiliation":[{"name":"Pediatric Infections Research Center Department of Pediatrics Mofid Children Hospital Shaheed Beheshti University of Medical Sciences and Health Services Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Zamani","sequence":"additional","affiliation":[{"name":"Department of Neurogenetics Iranian Centre of Neurological Research Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. H.","family":"Emami","sequence":"additional","affiliation":[{"name":"Poursina Hakim Research Institute Isfahan University of Medical Science Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,11]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"687","article-title":"Contribution of the MHC region to the familial risk of coeliac disease","volume":"36","author":"Bevan S.","year":"1999","journal-title":"Journal of Medical Genetics"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mpg.0000189337.08139.83"},{"key":"e_1_2_6_4_1","first-page":"143","article-title":"PCR\u2013sequence\u2010specific primer typing of HLA class I and class II alleles","volume":"210","author":"Bunce M.","year":"2003","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.11.1413"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.027"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.05.001"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00239-5"},{"key":"e_1_2_6_9_1","first-page":"141","article-title":"Diagnostic accuracy of IgA anti\u2010tissue transglutaminase in patients suspected of having celiac disease in Iran","volume":"17","author":"Emami M.H.","year":"2008","journal-title":"Journal of Gastrointestinal and Liver Diseases"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.50.5.624"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp001"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2009.04.001"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2007.01003.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.012"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e31821a23d0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.008"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365520701452217"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldn044"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1215"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-200003000-00005"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i15.2153"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2007.08.013"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e3181615ca7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-199910000-00019"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i41.6585"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-200305000-00003"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(93)90912-V"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004836-200303000-00007"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2001.057006540.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8628(19980305)76:2<183::AID-AJMG12>3.0.CO;2-H"}],"container-title":["International Journal of Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiji.12128","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.12128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T08:36:54Z","timestamp":1696322214000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iji.12128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,11]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/iji.12128"],"URL":"https:\/\/doi.org\/10.1111\/iji.12128","archive":["Portico"],"relation":{},"ISSN":["1744-3121","1744-313X"],"issn-type":[{"value":"1744-3121","type":"print"},{"value":"1744-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,11]]}}}