{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:16:09Z","timestamp":1747185369793,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T00:00:00Z","timestamp":1643241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HLA\u2010DQB2 is a gene of limited polymorphism, with unknown function that presents at least two transcript variants: v1, which encodes the full\u2010length beta\u2010chain, and v2, which lacks exon 4 and could give rise to a soluble protein. We previously showed a strong correlation between high v2 expression in preimplantation biopsies (PIB) of kidneys from young (18\u2010 to 49\u2010year olds) but not from old, deceased donors and 1\u2010year posttransplant low (estimated glomerular filtration rate\u00a0&lt;\u00a045\u00a0ml\/min\/1.73 m<jats:sup>2<\/jats:sup>) graft function (GF). In this study, we aimed to investigate the impact of posttransplant soluble HLA\u2010DQB2 (sDQB2) serum levels, v1 expression in PIB, and recipient HLA\u2010DQB2 rs7453920 A\/G polymorphism on GF. sDQB2 was evaluated by enzyme\u2010linked immunosorbent assay in sera from 114 recipients, collected at least 1 year (median 2.1 years) after transplantation. Higher sDQB2 levels were observed in recipients of kidneys from young, but not from old, donors that had a \u226530% decline in GF within 1 year after blood collection for sDQB2 determination. Among the 15 recipients of kidneys from young donors with sDQB2 \u2265 1.52\u00a0ng\/ml, 40% presented a \u226530% decline in GF, whereas this occurred in none of the 43 recipients with lower sDQB2 levels (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.007; OR: 36.5). Expression of HLA\u2010DQB2 variant 1, measured by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) in 92 PIB from young or old donors, did not significantly differ between transplants with high or low 4\u2010year GF. HLA\u2010DQB2 rs7453920 single nucleotide polymorphism (SNP) frequencies did not significantly differ between recipients with low or high 4\u2010year GF. We conclude that HLA\u2010DQB2 variant 1 expression in PIB and recipient rs7453920 SNP polymorphism are not associated with graft outcome. On the other hand, the association, in transplants of kidneys from young donors, between high posttransplant serum sDQB2 levels and decline in GF is a very interesting finding that deserves a validation study in a larger cohort.<\/jats:p>","DOI":"10.1111\/iji.12569","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T09:52:36Z","timestamp":1643277156000},"page":"63-69","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High soluble HLA\u2010DQB2 levels in posttransplant serum are associated with kidney graft dysfunction"],"prefix":"10.1111","volume":"49","author":[{"given":"Karina L","family":"Mine","sequence":"first","affiliation":[{"name":"Instituto de Imunogen\u00e9tica Associa\u00e7\u00e3o Fundo de Incentivo \u00e0 Pesquisa  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato","family":"de Marco","sequence":"additional","affiliation":[{"name":"Instituto de Imunogen\u00e9tica Associa\u00e7\u00e3o Fundo de Incentivo \u00e0 Pesquisa  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamiris R.F.","family":"Raimundo","sequence":"additional","affiliation":[{"name":"Instituto de Imunogen\u00e9tica Associa\u00e7\u00e3o Fundo de Incentivo \u00e0 Pesquisa  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia V.","family":"Ernesto","sequence":"additional","affiliation":[{"name":"Instituto de Imunogen\u00e9tica Associa\u00e7\u00e3o Fundo de Incentivo \u00e0 Pesquisa  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 O.","family":"Medina\u2010Pestana","sequence":"additional","affiliation":[{"name":"Nephrology Division, Hospital do Rim Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9lio","family":"Tedesco\u2010Silva","sequence":"additional","affiliation":[{"name":"Nephrology Division, Hospital do Rim Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3616-4579","authenticated-orcid":false,"given":"Maria","family":"Gerbase\u2010DeLima","sequence":"additional","affiliation":[{"name":"Instituto de Imunogen\u00e9tica Associa\u00e7\u00e3o Fundo de Incentivo \u00e0 Pesquisa  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000696"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00717908"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099724"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015050524"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2018.06.019"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00093"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002026"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"2530","DOI":"10.4049\/jimmunol.149.7.2530","article-title":"Conservation of the HLA\u2010DQB2 locus in nonhuman primates","volume":"149","author":"Gaur L. 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