{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T14:42:08Z","timestamp":1780065728686,"version":"3.54.0"},"reference-count":8,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009571","name":"Universidad Nacional de San Luis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009571","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA\u2010DQB1*02 allele is the main predisposing genetic factor and a candidate for first\u2010line genotyping screening. We designed and validated a simple, DNA purification\u2010free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and\/or epidemiological studies.<\/jats:p>","DOI":"10.1111\/iji.12656","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T22:44:46Z","timestamp":1706741086000},"page":"89-92","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Saliva direct PCR protocol for HLA\u2010DQB1*02 genotyping"],"prefix":"10.1111","volume":"51","author":[{"given":"Angeles","family":"Carrillo","sequence":"first","affiliation":[{"name":"Laboratorio de Desarrollo de Diagn\u00f3sticos Moleculares \u00c1rea Biolog\u00eda Molecular Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis  San Luis Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jimena","family":"Manzur","sequence":"additional","affiliation":[{"name":"Laboratorio de Desarrollo de Diagn\u00f3sticos Moleculares \u00c1rea Biolog\u00eda Molecular Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis  San Luis Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5787-0993","authenticated-orcid":false,"given":"Maximiliano","family":"Juri Ayub","sequence":"additional","affiliation":[{"name":"Laboratorio de Desarrollo de Diagn\u00f3sticos Moleculares \u00c1rea Biolog\u00eda Molecular Facultad de Qu\u00edmica Bioqu\u00edmica y Farmacia Universidad Nacional de San Luis  San Luis Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina55050190"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2017.307"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010018\u20100054\u2010z"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2008.10.018"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12427"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12441"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v26.i12.1365"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"}],"container-title":["International Journal of Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.12656","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T09:46:15Z","timestamp":1710236775000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iji.12656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,31]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1111\/iji.12656"],"URL":"https:\/\/doi.org\/10.1111\/iji.12656","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.10.25.23297535","asserted-by":"object"}]},"ISSN":["1744-3121","1744-313X"],"issn-type":[{"value":"1744-3121","type":"print"},{"value":"1744-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,31]]},"assertion":[{"value":"2023-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}