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For highly sensitised patients with a lower likelihood of finding a compatible donor, de\u2010listing of unacceptable antigens is an option to release organ offers. In this study, 164 serum samples from 106 potential renal transplant recipients were tested using HISTO SPOT HLA AB in parallel with testing by LABScreen Single Antigen (One Lambda) and cross\u2010matching by both CDC and flow cytometry. The results were analysed to assess the ability of HISTO SPOT HLA AB to predict a cross\u2010match result and to understand the relative sensitivity of this test compared with other available assays. 136 samples analysed were positive for donor\u2010specific antibodies (DSAs) using HISTO SPOT HLA AB. Of these, 17 (12.5%) were CDC positive, and 82 (60.3%) were positive by flow cytometry. A total of 28 sera which were negative for DSAs using HISTO SPOT HLA AB were negative by CDC and 25 (89.3%) were also flow cytometry cross\u2010match negative. In this early study, HISTO SPOT HLA AB has a 100% negative predictive value for CDC and 89.3% for flow cytometry cross\u2010matching. HISTO SPOT may therefore prove a useful additional tool to inform de\u2010listing strategies and to facilitate transplantation in highly sensitised patients.<\/jats:p>","DOI":"10.1111\/iji.12710","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T10:34:16Z","timestamp":1742207656000},"page":"135-140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of HISTO SPOT HLA AB With Cross\u2010Match Results"],"prefix":"10.1111","volume":"52","author":[{"given":"Madeleine R.","family":"Harris","sequence":"first","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester University NHS Foundation Trust  Manchester UK"},{"name":"Faculty of Biology Medicine and Health Division of Medical Education School of Medical Sciences University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Canterbury","sequence":"additional","affiliation":[{"name":"MC Diagnostics Ltd  Bromborough UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith E.","family":"Worthington","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester University NHS Foundation Trust  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus P.","family":"Lowe","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester University NHS Foundation Trust  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1345-3681","authenticated-orcid":false,"given":"Marie E.","family":"Hampson","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester University NHS Foundation Trust  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5608-8107","authenticated-orcid":false,"given":"Kay V.","family":"Poulton","sequence":"additional","affiliation":[{"name":"Transplantation Laboratory Manchester Royal Infirmary Manchester University NHS Foundation Trust  Manchester UK"},{"name":"Faculty of Biology Medicine and Health Division of Medical Education School of Medical Sciences University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2015.03.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_222_16"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12641"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.13913"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2020.03.003"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2019.12.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(00)02108-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trre.2013.07.001"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/iji.12658"},{"issue":"1","key":"e_1_2_7_11_1","first-page":"D783","article-title":"Allele Frequency Net Database (AFND) 2020 Update: Gold\u2010Standard Data Classification, Open Access Genotype Data and New Query Tools","volume":"48","author":"Gonzalez\u2010Galarza F. 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