{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:35:02Z","timestamp":1780407302060,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    IgA nephropathy (IgAN) is one of the most prevalent glomerulonephropathies and commonly leads to kidney failure. The recurrence of IgAN following transplantation remains a significant concern. Currently, detecting IgAN recurrence requires a kidney biopsy, highlighting the need for non\u2010invasive biomarkers such as donor\u2010derived cell\u2010free DNA (dd\u2010cfDNA) to aid in early detection. This prospective pilot study aims to evaluate dd\u2010cfDNA as a non\u2010invasive biomarker for detecting IgAN recurrence post\u2013kidney transplantation. Specifically, the study seeks to compare %dd\u2010cfDNA levels in transplanted patients with and without IgAN recurrence and correlate these levels with other kidney function parameters. A total of 32 patients with histologically confirmed IgAN were enrolled, including those with documented IgAN recurrence post\u2010transplantation and those without recurrence. Plasma samples were collected and processed using the AlloSeq cfDNA kit to quantify relative %dd\u2010cfDNA levels. Kidney function parameters, including estimated glomerular filtration rate (eGFR) and proteinuria, were also assessed. The study found no significant difference in %dd\u2010cfDNA levels between transplanted patients with IgAN recurrence and those without recurrence (median 0.37% [IQR 0.28%\u20133.53%] vs. median 0.42% [IQR 0.15%\u20130.84%],\n                    <jats:italic>p =<\/jats:italic>\n                    0.67). Also, %dd\u2010cfDNA (AUC = 0.57 [95% CI 0.28\u20130.85],\n                    <jats:italic>p =<\/jats:italic>\n                    0.64) failed to effectively discriminate IgAN recurrence compared to traditional kidney function parameters such as proteinuria (AUC = 0.96 [95% CI 0.87\u20131.00],\n                    <jats:italic>p =<\/jats:italic>\n                    0.002) and eGFR (AUC = 0.74 [95% CI 0.47\u20131.00],\n                    <jats:italic>p =<\/jats:italic>\n                    0.09). Relative (%) dd\u2010cfDNA alone may not be a robust biomarker for detecting IgAN recurrence post\u2010transplantation. While proteinuria proved a more effective indicator in this study, kidney biopsy remains the gold standard for definitive diagnosis. These findings highlight the challenges of using %dd\u2010cfDNA as a standalone diagnostic tool for monitoring IgAN recurrence post\u2010transplantation. Future research should explore larger patient cohorts and longitudinal assessments to refine the utility of dd\u2010cfDNA and investigate potential combination strategies with other biomarkers.\n                  <\/jats:p>","DOI":"10.1111\/iji.70010","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T08:13:09Z","timestamp":1756368789000},"page":"342-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Prospective Pilot Study to Investigate Whether Donor\u2010Derived Cell\u2010Free DNA Can Be Used as a Biomarker of Recurrent IgA Nephropathy Post\u2013Kidney Transplantation"],"prefix":"10.1111","volume":"52","author":[{"given":"Judith Owusuwaah","family":"Asiedu\u2010Basoah","sequence":"first","affiliation":[{"name":"Transplant Laboratory University Hospitals of Leicester  Leicester UK"},{"name":"The University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Weston","sequence":"additional","affiliation":[{"name":"Transplant Laboratory University Hospitals of Leicester  Leicester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Barratt","sequence":"additional","affiliation":[{"name":"Transplant Laboratory University Hospitals of Leicester  Leicester UK"},{"name":"Department of Cardiovascular Sciences University of Leicester  Leicester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Szklarzewicz","sequence":"additional","affiliation":[{"name":"Renal Research Department University Hospitals of Leicester  Leicester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Dunn","sequence":"additional","affiliation":[{"name":"Transplant Laboratory University Hospitals of Leicester  Leicester UK"},{"name":"TBGBiotechnology  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2023.07.011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000453081"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2015.158"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2016060670"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399\u20100012.2011.01424.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.11.034"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2021.101404"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196101"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/txd.0000000000001019"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20102277.2009.00966.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ckj\/sfaa060"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12882\u2010022\u201002802\u2010x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2021.04.009"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40472\u2010021\u201000349\u20108"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2013.04.019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16835"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000230131.66971.45"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15192"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2016.08.011"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15416"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.14066"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfr664"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2215\/cjn.11261114"},{"key":"e_1_2_9_25_1","unstructured":"Poulton K. 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