{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:50:17Z","timestamp":1789696217728,"version":"4.0.1"},"reference-count":4,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:00:00Z","timestamp":1773446400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunogenetics"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Accurate HLA allele identification is essential to ensure graft\u2010recipient compatibility. Advances in next\u2010generation sequencing (NGS), such as those provided by Illumina and Oxford Nanopore Technologies, have improved resolution of HLA\u2010typing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>Here, a novel HLA\u2010C*12 allele with a silent mutation (G to A) was identified in a bone marrow donor homozygous for HLA\u2010C*12. The allele was initially linked to HLA\u2010C*12:03:01 by short\u2010read NGS, but further investigation by long\u2010read sequencing revealed the mutation to be associated with HLA\u2010C*12:02:02. Phasing limitations of short\u2010read NGS made accurate allele assignment difficult, but precise differentiation became possible with longer reads. The origin of the mutation was subsequently confirmed by sequencing parental samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This case highlights the ability of long\u2010read sequencing to resolve cis\u2010trans ambiguities and improve allele phasing, and to enhance accuracy for new HLA allele identification.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iji.70046","type":"journal-article","created":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T07:07:22Z","timestamp":1773472042000},"page":"298-301","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Long\u2010Read Next\u2010Generation Sequencing Technologies Can Address Some Limitations of Short\u2010Read Technologies in HLA Typing"],"prefix":"10.1111","volume":"53","author":[{"given":"Julien","family":"Lion","sequence":"first","affiliation":[{"name":"Department of Histocompatibility Amiens University Medical Centre  Amiens France"},{"name":"EA HEMATIM 4666 Jules Verne University of Picardie  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Perriere","sequence":"additional","affiliation":[{"name":"Department of Histocompatibility Amiens University Medical Centre  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Desoutter","sequence":"additional","affiliation":[{"name":"Department of Histocompatibility Amiens University Medical Centre  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Guillaume","sequence":"additional","affiliation":[{"name":"Department of Histocompatibility Amiens University Medical Centre  Amiens France"},{"name":"EA HEMATIM 4666 Jules Verne University of Picardie  Amiens France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000538176"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tan.70440"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2020.04.009"}],"container-title":["International Journal of Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.70046","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iji.70046","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.70046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:58:58Z","timestamp":1777874338000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iji.70046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,14]]},"references-count":4,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/iji.70046"],"URL":"https:\/\/doi.org\/10.1111\/iji.70046","archive":["Portico"],"relation":{},"ISSN":["1744-3121","1744-313X"],"issn-type":[{"value":"1744-3121","type":"print"},{"value":"1744-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,14]]},"assertion":[{"value":"2025-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}