{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T12:09:29Z","timestamp":1789733369603,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Immunogenetics"],"abstract":"ABSTRACT<\/jats:title>\n \n \n Rheumatoid arthritis (RA) is closely associated with genetic factors, but there is still a lack of ideal biomarkers for its early diagnosis and precise intervention.<\/jats:p>\n <\/jats:sec>\n \n \n \n To investigate the diagnostic value and synovial cell function of miR\u20107641 in RA, and its mechanistic link to\n TNFSF10<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n \n \n A case\u2010control study was conducted with 152 RA patients and 152 matched asymptomatic first\u2010degree relatives. Serum miR\u20107641 and\n TNFSF10<\/jats:italic>\n levels were quantified by qRT\u2010PCR. Diagnostic accuracy was assessed via logistic regression and ROC analysis. In MH7A cells, miR\u20107641 mimic\/inhibitor transfection was performed, followed by assessments of proliferation (CCK\u20108), inflammatory cytokines (ELISA), and target interaction (dual\u2010luciferase assay). Rescue experiments involved co\u2010transfection of the miR\u20107641 mimic and a\n TNFSF10<\/jats:italic>\n overexpression plasmid.\n <\/jats:p>\n <\/jats:sec>\n \n \n \n Serum miR\u20107641 was downregulated in RA patients and identified as an independent protective factor with high diagnostic accuracy (AUC\u00a0=\u00a00.902). In MH7A cells, miR\u20107641 overexpression suppressed proliferation and reduced IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1 secretion.\n TNFSF10<\/jats:italic>\n was confirmed as a direct target, with its expression negatively correlating with miR\u20107641 in serum samples. Importantly,\n TNFSF10<\/jats:italic>\n overexpression rescued the inhibitory effects of miR\u20107641 on proliferation and cytokine production.\n <\/jats:p>\n <\/jats:sec>\n \n \n \n This study identifies serum miR\u20107641 as a potential diagnostic biomarker for RA. In an immortalized synovial cell model, miR\u20107641 exerts protective effects by targeting\n TNFSF10<\/jats:italic>\n to inhibit proliferation and inflammation. Future studies in primary cells and in vivo models are required to validate these findings and explore downstream signalling mechanisms.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/iji.70065","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:44:59Z","timestamp":1789731899000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR\u20107641: A Novel Diagnostic Biomarker for Rheumatoid Arthritis That Exerts Protective Effects by Targeting\n TNFSF10<\/i>"],"prefix":"10.1111","author":[{"given":"Yuchen","family":"Zhang","sequence":"first","affiliation":[{"name":"MRI Room, Suixi County People's Hospital Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Radiology Pukou Hospital of Traditional Chinese Medicine Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongming","family":"Liang","sequence":"additional","affiliation":[{"name":"Changsha Central Hospital Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery and Geriatric Orthopedics The Affiliated Hospital of Youjiang Medical University for Nationalities Baise China"},{"name":"Guangxi Key Laboratory for Preclinical and Translational Research on Bone and Joint Degenerative Diseases Baise China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Fengdu General Hospital Fengdu Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"issue":"11","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1111\/ijcp.14651","article-title":"Serum miR\u2010224, miR\u2010760, miR\u2010483\u20105p, miR\u2010378 and miR\u2010375 as Potential Novel Biomarkers in Rheumatoid Arthritis","volume":"75","author":"Abdelaleem O. O.","year":"2021","journal-title":"International Journal of Clinical Practice"},{"issue":"9","key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1002\/art.27584","article-title":"2010 Rheumatoid Arthritis Classification Criteria: An American College of Rheumatology\/European League Against Rheumatism Collaborative Initiative","volume":"62","author":"Aletaha D.","year":"2010","journal-title":"Arthritis & Rheumatism"},{"issue":"18","key":"e_1_2_9_4_1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms251810135","article-title":"Computational Screening to Predict MicroRNA Targets in the Flavivirus 3\u2032 UTR Genome: An Approach for Antiviral Development","volume":"25","author":"Avila\u2010Bonilla R. G.","year":"2024","journal-title":"International Journal of Molecular Sciences"},{"issue":"6","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.3390\/ani13061046","article-title":"PDZK1\u2010Interacting Protein 1 (PDZKIP1) Inhibits Goat Subcutaneous Preadipocyte Differentiation Through Promoting Autophagy","volume":"13","author":"Chen D.","year":"2023","journal-title":"Animals"},{"issue":"10391","key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1016\/S0140-6736(23)00457-9","article-title":"Incidence, Prevalence, and Co\u2010Occurrence of Autoimmune Disorders Over Time and by Age, Sex, and Socioeconomic Status: A Population\u2010Based Cohort Study of 22 Million Individuals in the UK","volume":"401","author":"Conrad N.","year":"2023","journal-title":"Lancet"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.3389\/fimmu.2019.02449","article-title":"Inhibition of miR\u2010378a\u20103p by Inflammation Enhances IL\u201033 Levels: A Novel Mechanism of Alarmin Modulation in Ulcerative Colitis","volume":"10","author":"Dubois\u2010Camacho K.","year":"2019","journal-title":"Frontiers in Immunology"},{"issue":"1","key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"4991","DOI":"10.1038\/s41467-024-49186-0","article-title":"Clonal Associations Between Lymphocyte Subsets and Functional States in Rheumatoid Arthritis Synovium","volume":"15","author":"Dunlap G.","year":"2024","journal-title":"Nature Communications"},{"issue":"2","key":"e_1_2_9_9_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1012061","article-title":"Downregulation of CPSF6 Leads to Global mRNA 3'UTR Shortening and Enhanced Antiviral Immune Responses","volume":"20","author":"Ge Y.","year":"2024","journal-title":"PLoS Pathogens"},{"issue":"1","key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/s41413-025-00434-z","article-title":"IGF2BP3\u2010Mediated m6A Modification of RASGRF1 Promoting Joint Injury in Rheumatoid Arthritis","volume":"13","author":"Geng Q.","year":"2025","journal-title":"Bone Research"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.prp.2023.154960","article-title":"Hsa\u2010miR\u201021\u20135p Reflects Synovitis and Tenosynovitis Components of Musculoskeletal Ultrasonography Seven\u2010Joint Scores in Rheumatoid Arthritis Disease and Predicts the Disease Flare","volume":"253","author":"Hammad R.","year":"2024","journal-title":"Pathology\u2014Research and Practice"},{"issue":"1","key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"4508","DOI":"10.1038\/s41598-023-31438-6","article-title":"Bioinformatics Analysis of Rheumatoid Arthritis Tissues Identifies Genes and Potential Drugs That are Expressed Specifically","volume":"13","author":"He Q.","year":"2023","journal-title":"Scientific Reports"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.tice.2021.101591","article-title":"The Effects of miR\u201026b\u20105p on Fibroblast\u2010Like Synovial Cells in Rheumatoid Arthritis (RA\u2010FLS) via Targeting EZH2","volume":"72","author":"Hu P.","year":"2021","journal-title":"Tissue and Cell"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2024.1374843","article-title":"Regulatory Role of RNA\u2010Binding Proteins in microRNA Biogenesis","volume":"11","author":"Hynes C.","year":"2024","journal-title":"Frontiers in Molecular Biosciences"},{"issue":"4","key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41580-024-00805-0","article-title":"The Biogenesis and Regulation of Animal microRNAs","volume":"26","author":"Kim H.","year":"2025","journal-title":"Nature Reviews Molecular Cell Biology"},{"issue":"5","key":"e_1_2_9_16_1","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.7150\/thno.93115","article-title":"Extracellular Vesicles as Carriers of mRNA: Opportunities and Challenges in Diagnosis and Treatment","volume":"14","author":"Kirian R. 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M.","year":"2025"},{"issue":"2","key":"e_1_2_9_31_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/bjd.21232","article-title":"miR\u2010378a Regulates Keratinocyte Responsiveness to Interleukin\u201017A in Psoriasis","volume":"187","author":"Xia P.","year":"2022","journal-title":"British Journal of Dermatology"},{"issue":"13","key":"e_1_2_9_32_1","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.7150\/jca.86735","article-title":"TNFSF10, an Autophagy Related Gene, Was a Prognostic and Immune Infiltration Marker in Skin Cutaneous Melanoma","volume":"14","author":"Xue L.","year":"2023","journal-title":"Journal of Cancer"},{"issue":"21","key":"e_1_2_9_33_1","doi-asserted-by":"crossref","DOI":"10.1172\/JCI170706","article-title":"GLUT3 Promotes Macrophage Signaling and Function via RAS\u2010Mediated Endocytosis in Atopic Dermatitis and Wound Healing","volume":"133","author":"Yu D.\u2010M.","year":"2023","journal-title":"Journal of Clinical Investigation"},{"issue":"14","key":"e_1_2_9_34_1","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202501452R","article-title":"TNF\u2010\u03b1 Promotes Synovial Inflammation and Cartilage Bone Destruction in Rheumatoid Arthritis via NF\u2010\u03baB\/YY1\/miR\u2010103a\u20103p Axis","volume":"39","author":"Yuan Y.","year":"2025","journal-title":"FASEB Journal"},{"key":"e_1_2_9_35_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2022.115837","article-title":"Gancao Fuzi Decoction Regulates the Th17\/Treg Cell Imbalance in Rheumatoid Arthritis by Targeting Foxp3 via miR\u201034a","volume":"301","author":"Zhao X.","year":"2023","journal-title":"Journal of Ethnopharmacology"},{"issue":"4","key":"e_1_2_9_36_1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1080\/15592294.2019.1676613","article-title":"Identification of PBMC\u2010Expressed miRNAs for Rheumatoid Arthritis","volume":"15","author":"Zhu X.","year":"2020","journal-title":"Epigenetics"}],"container-title":["International Journal of Immunogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.70065","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iji.70065","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iji.70065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:45:04Z","timestamp":1789731904000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iji.70065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":35,"alternative-id":["10.1111\/iji.70065"],"URL":"https:\/\/doi.org\/10.1111\/iji.70065","archive":["Portico"],"relation":{},"ISSN":["1744-3121","1744-313X"],"issn-type":[{"value":"1744-3121","type":"print"},{"value":"1744-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70065"}}