{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T05:23:56Z","timestamp":1696397036702},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,11,28]],"date-time":"2012-11-28T00:00:00Z","timestamp":1354060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":1007,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Summary<\/jats:title>Introduction<\/jats:title>This is the first pilot study to screen multiple common genetic aberrations in B<\/jats:styled-content>\u2010cell precursor acute lymphoblastic leukemia (BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Thirty\u2010two children with BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content> were investigated for chromosomal rearrangements using interphase fluorescence in situ<\/jats:italic> hybridization (FISH<\/jats:styled-content>). Eight common translocations and rearrangements, including ETV<\/jats:styled-content>6\u2010RUNX<\/jats:styled-content>1<\/jats:italic>, TCF<\/jats:styled-content>3\u2010PBX<\/jats:styled-content>1<\/jats:italic>, BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content>1<\/jats:italic>, ETV<\/jats:styled-content>6<\/jats:italic>, TCF<\/jats:styled-content>3<\/jats:italic>, MLL<\/jats:styled-content><\/jats:italic>, IGH<\/jats:styled-content>@<\/jats:italic>, and PAX<\/jats:styled-content>5<\/jats:italic>, were tested for using dual\u2010color DNA<\/jats:styled-content> probes.<\/jats:p><\/jats:sec>Results<\/jats:title>ETV<\/jats:styled-content>6\u2010RUNX<\/jats:styled-content>1<\/jats:italic> was the most frequent translocation detected in 11 children (34.4%). Two patients with BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content>1<\/jats:italic> (6.3%) and one with TCF<\/jats:styled-content>3\u2010PBX<\/jats:styled-content>1<\/jats:italic> (3.1%) translocations were also observed. Using break\u2010apart probes, 11 children (34.4%) had a positive FISH<\/jats:styled-content> result for ETV<\/jats:styled-content>6<\/jats:italic>, two patients for IGH<\/jats:styled-content>@<\/jats:italic> (6.3%), one patient for MLL<\/jats:styled-content><\/jats:italic> (3.1%), and one patient for PAX<\/jats:styled-content>5<\/jats:italic> rearrangements (3.1%). All patients with the ETV<\/jats:styled-content>6\u2010RUNX<\/jats:styled-content>1<\/jats:italic> fusion were also identified by split signals for ETV<\/jats:styled-content>6<\/jats:italic>. Other abnormalities, including extra copies and deletion of genes, were observed within the range of 3.1\u201334.4%. Cytogenetics analysis showed a single case each of BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content>1<\/jats:italic> fusion, MLL<\/jats:styled-content>,<\/jats:italic> and IGH<\/jats:styled-content>@<\/jats:italic> rearrangements (3.1% each). ETV<\/jats:styled-content>6\u2010RUNX<\/jats:styled-content>1<\/jats:italic> fusion and ETV<\/jats:styled-content>6<\/jats:italic> split\u2010apart rearrangements were not visible by cytogenetics. Likewise, one each of cases with TCF<\/jats:styled-content>3\u2010PBX<\/jats:styled-content>1<\/jats:italic> fusion and with PAX<\/jats:styled-content>5<\/jats:italic> split signal seen by FISH<\/jats:styled-content> was not visible by cytogenetics.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>By using 8 FISH<\/jats:styled-content> probes in conjunction cytogenetics for the detection of common aberrations, interphase FISH<\/jats:styled-content> enhanced the detection of chromosomal rearrangements in children with BCP<\/jats:styled-content>\u2010ALL<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijlh.12031","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T00:50:52Z","timestamp":1354150252000},"page":"406-415","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Interphase\u2010FISH<\/scp> screening for eight common rearrangements in pediatric B<\/scp>\u2010cell 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